Photochemical mechanisms of fluorophores employed in single‐molecule localization microscopy

K Kikuchi, LD Adair, J Lin, EJ New… - Angewandte Chemie, 2023‏ - Wiley Online Library
Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Rejuvenating old fluorophores with new chemistry

MJ Schnermann, LD Lavis - Current opinion in chemical biology, 2023‏ - Elsevier
The field of organic chemistry began with 19th century scientists identifying and then
expanding upon synthetic dye molecules for textiles. In the 20th century, dye chemistry …

Method to diversify cyanine chromophore functionality enables improved biomolecule tracking and intracellular imaging

SM Usama, SC Marker, DH Li… - Journal of the …, 2023‏ - ACS Publications
Heptamethine indocyanines are invaluable probes for near-infrared (NIR) imaging. Despite
broad use, there are only a few synthetic methods to assemble these molecules, and each …

The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes

PR Steen, EM Unterauer, LA Masullo, J Kwon… - Nature …, 2024‏ - nature.com
DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-
resolution fluorescence microscopy technique that achieves single-molecule 'blinking'by …

Heptamethine cyanine dyes with ultra‐efficient excited‐state nonradiative decay for synergistic photothermal immunotherapy

X **a, C Shi, S He, R Wang, Z Zhang… - Advanced Functional …, 2023‏ - Wiley Online Library
Organic dyes in the excited singlet state (S1) decay via intersystem crossing (ISC) to the
triplet state, radiative dissipation as fluorescence, or nonradiative decay for thermal …

[HTML][HTML] Recent advances in minimal fluorescent probes for optical imaging

F De Moliner, F Nadal-Bufi, M Vendrell - Current opinion in chemical …, 2024‏ - Elsevier
Fluorescent probes have revolutionized biological imaging by enabling the real-time
visualization of cellular processes under physiological conditions. However, their size and …

Recent advances in the development of fluorescent probes for γ‐glutamyltranspeptidase

ZY Hu, XY Chen, YS Yang, SJ Wang, ZG Hu… - Coordination Chemistry …, 2024‏ - Elsevier
Abstract γ-Glutamyltranspeptidase (GGT) plays a significant role in the regulation of cellular
redox homeostasis and other physiological or pathophysiological processes. The over …

Aryl‐Modified Pentamethyl Cyanine Dyes at the C2'Position: A Tunable Platform for Activatable Photosensitizers

F Han, SA Abbas Abedi, S He, H Zhang… - Advanced …, 2024‏ - Wiley Online Library
Pentamethyl cyanine dyes are promising fluorophores for fluorescence sensing and
imaging. However, advanced biomedical applications require enhanced control of their …

Small fluorogenic amino acids for peptide‐guided background‐free imaging

F De Moliner, Z Konieczna… - Angewandte Chemie …, 2023‏ - Wiley Online Library
The multiple applications of super‐resolution microscopy have prompted the need for
minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many …

Single-molecule FRET at 10 MHz count rates

L Grabenhorst, F Sturzenegger, M Hasler… - Journal of the …, 2024‏ - ACS Publications
A bottleneck in many studies utilizing single-molecule Förster resonance energy transfer is
the attainable photon count rate, as it determines the temporal resolution of the experiment …