The steady progress in genome editing, especially genome editing based on the use of
clustered regularly interspaced short palindromic repeats (CRISPR) and programmable …

Malaria caused by Plasmodium is still a serious public health problem. Genomic editing is
essential to understand parasite biology, elucidate mechanical pathways, uncover gene …

The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins.
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …

Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by
activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound …

Abstract CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-
stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional …

Non-specific amplification is a major problem in nucleic acid amplification resulting in false-
positive results, especially for exponential amplification reactions (EXPAR). Although efforts …

Various genome editing tools have been developed for programmable genome
manipulation at specified genomic loci. However, it is crucial to comprehensively interrogate …

CRISPR-Cas12a is an alternative class 2 gene editing tool that may cause less off-target
effects than the original Cas9 system. We have previously demonstrated that Cas12a attack …

CRISPR-Cas systems play a critical role in the prokaryotic adaptive immunity against mobile
genetic elements, such as phages and foreign plasmids. In the last decade, Cas9 has been …

CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA
containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a …