Inteins as drug targets and therapeutic tools

AM Tharappel, Z Li, H Li - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Multidrug-resistant pathogens are of significant concern in recent years. Hence new
antifungal and anti-bacterial drug targets are urgently needed before the situation goes …

Inteins: A Swiss army knife for synthetic biology

S Anastassov, M Filo, M Khammash - Biotechnology Advances, 2024 - Elsevier
Inteins are proteins found in nature that execute protein splicing. Among them, split inteins
stand out for their versatility and adaptability, presenting creative solutions for addressing …

Metal regulation of Mycobacterium tuberculosis SufB intein splicing at the host–pathogen crossroad

S Panda, A Nanda, SS Nasker, A Mehra… - Clinical …, 2023 - portlandpress.com
Intein sequences self-excise from precursor proteins to generate functional proteins in
various organisms. Thus, regulation of intein splicing at the host–pathogen interface can …

Intein inhibitors as novel antimicrobials: protein splicing in human pathogens, screening methods, and off-target considerations

DA Wall, SP Tarrant, C Wang, KV Mills… - Frontiers in Molecular …, 2021 - frontiersin.org
Protein splicing is a post-translational process by which an intervening polypeptide, or
intein, catalyzes its own removal from the flanking polypeptides, or exteins, concomitant with …

Calcimycin Inhibits Cryptococcus neoformans In Vitro and In Vivo by Targeting the Prp8 Intein Splicing

AM Tharappel, Z Li, YC Zhu, X Wu… - ACS infectious …, 2022 - ACS Publications
Drug resistance is a significant concern in the treatment of diseases, including
cryptococcosis caused by Cryptococcus neoformans (Cne) and Cryptococcus gattii (Cga) …

SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines

S Panda, A Nanda, N Sahu, DK Ojha… - Bioscience …, 2022 - portlandpress.com
Inteins are auto-processing domains that implement a multistep biochemical reaction termed
protein splicing, marked by cleavage and formation of peptide bonds. They excise from a …

Molecular docking and MD simulations reveal protease inhibitors block the catalytic residues in Prp8 intein of Aspergillus fumigatus: a potential target for antimycotics

S Panda, M Rout, S Mishra, J Turuk, S Pati… - Journal of …, 2023 - Taylor & Francis
Resistance to azoles and amphotericin B especially in Aspergillus fumigatus is a growing
concern towards the treatment of invasive fungal infection. At this critical juncture, intein …

Conditional protein splicing triggered by SUMO protease

M Xu, S Wang, Q Zhan, Y Lin - Biochemical and Biophysical Research …, 2023 - Elsevier
Conditional protein splicing is a powerful biotechnological tool that can be used to post-
translationally control the activity of target proteins. Here we demonstrated a novel …

Cleaving the way for heterologous peptide production: An overview of cleavage strategies

KO Osiro, HM Duque, KBS de Oliveira, NTM Melo… - Methods, 2024 - Elsevier
One of the main bottlenecks for recombinant peptide production is choosing the proper
cleavage method to remove fusion protein tags from target peptides. While these tags are …

Characteristics, Recombination Methods, and Applications Progresses of Split-Cas9 System

Z Liu, L Huang, H Deng, Y Chen, H **ao - Human Gene Therapy, 2023 - liebertpub.com
CRISPR technology has been used to revolutionize various facets of life sciences because
of its potent gene editing capabilities. In particular, CRISPR technology is anticipated to be …