Robust acquisition of spatial transcriptional programs in tissues with immunofluorescence-guided laser capture microdissection

X Zhang, C Hu, C Huang, Y Wei, X Li, M Hu… - Frontiers in Cell and …, 2022 - frontiersin.org
The functioning of tissues is fundamentally dependent upon not only the phenotypes of the
constituent cells but also their spatial organization in the tissue, as local interactions …

Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genoty**

JJ Credle, ML Robinson, J Gunn, D Monaco, B Sie… - Modern …, 2021 - nature.com
There is an urgent and unprecedented need for sensitive and high-throughput molecular
diagnostic tests to combat the SARS-CoV-2 pandemic. Here we present a generalized …

Pancreatic cancer driver mutations are targetable through distant alternative RNA splicing dependencies

RR Kawalerski, SD Leach, LF Escobar-Hoyos - Oncotarget, 2021 - pmc.ncbi.nlm.nih.gov
Pancreatic ductal adenocarcinoma (PDAC), the most common histological subtype of
pancreatic cancer, has one of the highest case fatality rates of all known solid malignancies …

Fluid delivery methods

H Kim, AM Tentori, S **ng, R Bharadwaj… - US Patent …, 2024 - Google Patents
Provided herein is a fluid delivery method for permeabilizing a biological sample. The
method includes delivering the fluid to a first substrate and/or a second substrate. At least …

Dual Probe Ligation In Situ Hybridization with Rolling-Circle Amplification for High-Plex Spatial Transcriptomics

S Maguire, J Credle, E Bertelson, S Lee, B Cha, D **e… - bioRxiv, 2025 - biorxiv.org
New biological insights are increasingly dependent upon a deeper understanding of tissue
architectures. Critical to such studies are spatial transcriptomics technologies, especially …

Robust acquisition of high resolution spatial transcriptomes from preserved tissues with immunofluorescence based laser capture microdissection

X Zhang, C Hu, C Huang, Y Wei, X Li, M Hu, H Li, J Wu… - bioRxiv, 2021 - biorxiv.org
The functioning of tissues is fundamentally dependent upon not only the phenotypes of the
constituent cells but also their spatial organization in the tissue. However, obtaining …

In situ rna analysis using probe pair ligation

H Larman, J Credie - US Patent App. 17/790,293, 2023 - Google Patents
The present invention relates to the field of ribonucleotide analysis. More specifically, the
present invention provides compositions and methods for detection for nucleic acids using …

Beyond the microscope: interpreting renal biopsy findings in the era of precision medicine

SM Bagnasco - American Journal of Physiology-Renal …, 2018 - journals.physiology.org
As rapid progress in science and biotechnology is affecting the practice of renal medicine,
increasingly precise diagnostic assessment is needed to select the most effective …

HybriSeq: Probe-based Device-free Single-cell RNA Profiling

D Foyt, D Brown, S Zhou, B Huang - bioRxiv, 2024 - pmc.ncbi.nlm.nih.gov
We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ
hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and …

[PDF][PDF] Optimized RT-qPCR Assays for Rapid and Reliable Detection of SARS

J King, S Whitmore, C Drayton - 2023 - researchgate.net
Authors: Jiam King, Samuel Whitmore, Charlotte Drayton Abstract: The rise of SARS-CoV-2
has created an urgent need for accurate diagnostic tools to control the spread of COVID-19 …