An updated evolutionary classification of CRISPR–Cas systems
KS Makarova, YI Wolf, OS Alkhnbashi… - Nature reviews …, 2015 - nature.com
The evolution of CRISPR–cas loci, which encode adaptive immune systems in archaea and
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence
Pseudomonas aeruginosa is an opportunistic pathogen affecting immunocompromised
patients. It is known as the leading cause of morbidity and mortality in cystic fibrosis (CF) …
patients. It is known as the leading cause of morbidity and mortality in cystic fibrosis (CF) …
Genome engineering using the CRISPR-Cas9 system
Targeted nucleases are powerful tools for mediating genome alteration with high precision.
The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short …
The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short …
Multiplex genome engineering using CRISPR/Cas systems
Functional elucidation of causal genetic variants and elements requires precise genome
editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short …
editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short …
[HTML][HTML] Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Targeted genome editing technologies have enabled a broad range of research and
medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted …
medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted …
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …
[HTML][HTML] Crystal structure of Cas9 in complex with guide RNA and target DNA
The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by
single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus …
single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus …
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
E Deltcheva, K Chylinski, CM Sharma, K Gonzales… - Nature, 2011 - nature.com
CRISPR/Cas systems constitute a widespread class of immunity systems that protect
bacteria and archaea against phages and plasmids, and commonly use repeat/spacer …
bacteria and archaea against phages and plasmids, and commonly use repeat/spacer …
Evolution and classification of the CRISPR–Cas systems
The CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-
associated proteins) modules are adaptive immunity systems that are present in many …
associated proteins) modules are adaptive immunity systems that are present in many …
Endoplasmic reticulum stress: molecular mechanism and therapeutic targets
X Chen, C Shi, M He, S **ong, X **a - Signal transduction and targeted …, 2023 - nature.com
The endoplasmic reticulum (ER) functions as a quality-control organelle for protein
homeostasis, or “proteostasis”. The protein quality control systems involve ER-associated …
homeostasis, or “proteostasis”. The protein quality control systems involve ER-associated …