Serving as the basis of cell life, interactions between nucleic acids and proteins play
essential roles in fundamental cellular processes. Aptamers are unique single-stranded …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

The α7 nicotinic acetylcholine receptor plays critical roles in the central nervous system and
in the cholinergic inflammatory pathway. This ligand-gated ion channel assembles as a …

Using cryo-electron microscopy (cryo-EM), we determined the structure of the Escherichia
coli 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous …

In the many scientific endeavors that are driven by organic chemistry, unambiguous
identification of small molecules is of paramount importance. Over the past 50 years, NMR …

In 2020, cryo-EM single-particle analysis achieved true atomic resolution thanks to
technological developments in hardware and software. The number of high-resolution …

Three-dimensional structure modeling from maps is an indispensable step for studying
proteins and their complexes with cryogenic electron microscopy. Although the resolution of …

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …

Human cytomegalovirus (HCMV) glycoprotein B (gB) is a class III membrane fusion protein
required for viral entry. HCMV vaccine candidates containing gB have demonstrated …

In this review, we describe recent research developments into radiation damage effects in
macromolecular X-ray crystallography observed at synchrotrons and X-ray free electron …