Novel CRISPR–Cas systems: an updated review of the current achievements, applications, and future research perspectives

S Nidhi, U Anand, P Oleksak, P Tripathi, JA Lal… - International journal of …, 2021 - mdpi.com
According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

CRISPR for crop improvement: an update review

D Jaganathan, K Ramasamy, G Sellamuthu… - Frontiers in plant …, 2018 - frontiersin.org
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …

Precise integration of large DNA sequences in plant genomes using PrimeRoot editors

C Sun, Y Lei, B Li, Q Gao, Y Li, W Cao, C Yang… - Nature …, 2024 - nature.com
A technique for chromosomal insertion of large DNA segments is much needed in plant
breeding and synthetic biology to facilitate the introduction of desired agronomic traits and …

Characterization and heterologous reconstitution of Taxus biosynthetic enzymes leading to baccatin III

B Jiang, L Gao, H Wang, Y Sun, X Zhang, H Ke, S Liu… - Science, 2024 - science.org
Paclitaxel is a well known anticancer compound. Its biosynthesis involves the formation of a
highly functionalized diterpenoid core skeleton (baccatin III) and the subsequent assembly …

Variation in TaSPL6-D confers salinity tolerance in bread wheat by activating TaHKT1;5-D while preserving yield-related traits

M Wang, J Cheng, J Wu, J Chen, D Liu, C Wang… - Nature Genetics, 2024 - nature.com
Na+ exclusion from above-ground tissues via the Na+-selective transporter HKT1; 5 is a
major salt-tolerance mechanism in crops. Using the expression genome-wide association …

Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors

B Al-Shayeb, P Skopintsev, KM Soczek, EC Stahl, Z Li… - Cell, 2022 - cell.com
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …

Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

OX Dong, S Yu, R Jain, N Zhang, PQ Duong… - Nature …, 2020 - nature.com
Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a
desirable alternative to insertions at random sites achieved through conventional methods …

Domestication of wild tomato is accelerated by genome editing

T Li, X Yang, Y Yu, X Si, X Zhai, H Zhang, W Dong… - Nature …, 2018 - nature.com
Crop improvement by inbreeding often results in fitness penalties and loss of genetic
diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by …

[HTML][HTML] Rhizosphere microbiome: Engineering bacterial competitiveness for enhancing crop production

A Kumar, A Dubey - Journal of Advanced Research, 2020 - Elsevier
Plants in nature are constantly exposed to a variety of abiotic and biotic stresses which limits
their growth and production. Enhancing crop yield and production to feed exponentially …

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

C Li, Y Zong, Y Wang, S **, D Zhang, Q Song… - Genome biology, 2018 - Springer
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine.
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …