Single-cell and spatial multi-omics in the plant sciences: Technical advances, applications, and perspectives

X Yu, Z Liu, X Sun - Plant communications, 2023 - cell.com
Plants contain a large number of cell types and exhibit complex regulatory mechanisms.
Studies at the single-cell level have gradually become more common in plant science …

Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants

C Chen, Y Ge, L Lu - Frontiers in Plant Science, 2023 - frontiersin.org
Single-cell and spatial transcriptomics have diverted researchers' attention from the
multicellular level to the single-cell level and spatial information. Single-cell transcriptomes …

Single-cell resolution of lineage trajectories in the Arabidopsis stomatal lineage and develo** leaf

CB Lopez-Anido, A Vatén, NK Smoot, N Sharma… - Developmental cell, 2021 - cell.com
Dynamic cell identities underlie flexible developmental programs. The stomatal lineage in
the Arabidopsis leaf epidermis features asynchronous and indeterminate divisions that can …

Dynamics of Gene Expression in Single Root Cells of Arabidopsis thaliana

K Jean-Baptiste, JL McFaline-Figueroa… - The plant …, 2019 - academic.oup.com
Single cell RNA sequencing can yield high-resolution cell-type–specific expression
signatures that reveal new cell types and the developmental trajectories of cell lineages …

Cell type–specific attenuation of brassinosteroid signaling precedes stomatal asymmetric cell division

EJ Kim, C Zhang, B Guo, T Eekhout… - Proceedings of the …, 2023 - National Acad Sciences
In Arabidopsis thaliana, brassinosteroid (BR) signaling and stomatal development are
connected through the SHAGGY/GSK3-like kinase BR INSENSITIVE2 (BIN2). BIN2 is a key …

CRISPR-TSKO: a technique for efficient mutagenesis in specific cell types, tissues, or organs in Arabidopsis

W Decaestecker, RA Buono, ML Pfeiffer… - The Plant …, 2019 - academic.oup.com
Detailed functional analyses of many fundamentally important plant genes via conventional
loss-of-function approaches are impeded by the severe pleiotropic phenotypes resulting …

A gene regulatory network for root epidermis cell differentiation in Arabidopsis

A Bruex, RM Kainkaryam, Y Wieckowski, YH Kang… - PLoS …, 2012 - journals.plos.org
The root epidermis of Arabidopsis provides an exceptional model for studying the molecular
basis of cell fate and differentiation. To obtain a systems-level view of root epidermal cell …

Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection

C Procko, T Lee, A Borsuk, BOR Bargmann… - The Plant …, 2022 - academic.oup.com
Like other complex multicellular organisms, plants are composed of different cell types with
specialized shapes and functions. For example, most laminar leaves consist of multiple …

The vacuolar manganese transporter MTP8 determines tolerance to iron deficiency-induced chlorosis in Arabidopsis

S Eroglu, B Meier, N von Wirén, E Peiter - Plant physiology, 2016 - academic.oup.com
Iron (Fe) deficiency is a widespread nutritional disorder on calcareous soils. To identify
genes involved in the Fe deficiency response, Arabidopsis (Arabidopsis thaliana) transfer …

The YTHDF proteins ECT2 and ECT3 bind largely overlap** target sets and influence target mRNA abundance, not alternative polyadenylation

L Arribas-Hernández, S Rennie, M Schon, C Porcelli… - Elife, 2021 - elifesciences.org
Gene regulation via N6-methyladenosine (m6A) in mRNA involves RNA-binding proteins
that recognize m6A via a YT521-B homology (YTH) domain. The plant YTH domain proteins …