Current and emerging trends in techniques for plant pathogen detection

M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …

High‐throughput identification and diagnostics of pathogens and pests: overview and practical recommendations

L Tedersoo, R Drenkhan, S Anslan… - Molecular ecology …, 2019 - Wiley Online Library
High‐throughput identification technologies provide efficient tools for understanding the
ecology and functioning of microorganisms. Yet, these methods have been only rarely used …

Next generation sequencing for detection and discovery of plant viruses and viroids: comparison of two approaches

A Pecman, D Kutnjak, I Gutiérrez-Aguirre… - Frontiers in …, 2017 - frontiersin.org
Next generation sequencing (NGS) technologies are becoming routinely employed in
different fields of virus research. Different sequencing platforms and sample preparation …

Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description

B Lebas, I Adams, M Al Rwahnih, S Baeyen… - EPPO …, 2022 - Wiley Online Library
High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous
detection and potential identification of any organisms present in a sample. The growing …

Insect-specific viruses: from discovery to potential translational applications

S Nouri, EE Matsumura, YW Kuo, BW Falk - Current opinion in virology, 2018 - Elsevier
Highlights•NGS and bioinformatics analyses show that the insect virosphere contains many
previously undiscovered viruses of many taxa.•Insect-specific viruses (ISVs) do not infect …

Comparative study on three viral enrichment approaches based on RNA extraction for plant virus/viroid detection using high-throughput sequencing

YZA Gaafar, H Ziebell - PLoS One, 2020 - journals.plos.org
High-throughput sequencing (HTS) has become increasingly popular as virus diagnostic
tool. It has been used to detect and identify plant viruses and viroids in different types of …

High-throughput detection of a large set of viruses and viroids of pome and stone fruit trees by multiplex PCR-based amplicon sequencing

LC Costa, B Atha III, X Hu, K Lamour, Y Yang… - Frontiers in Plant …, 2022 - frontiersin.org
A comprehensive diagnostic method of known plant viruses and viroids is necessary to
provide an accurate phytosanitary status of fruit trees. However, most widely used detection …

Targeted genome sequencing (TG-Seq) approaches to detect plant viruses

S Maina, L Zheng, BC Rodoni - Viruses, 2021 - mdpi.com
Globally, high-throughput sequencing (HTS) has been used for virus detection in germplasm
certification programs. However, sequencing costs have impeded its implementation as a …

Canine meningoencephalitis of unknown origin—the search for infectious agents in the cerebrospinal fluid via deep sequencing

JN Nessler, WK Jo, ADME Osterhaus… - Frontiers in Veterinary …, 2021 - frontiersin.org
Meningoencephalitis of unknown origin (MUO) describes a group of meningoencephalitides
in dogs with a hitherto unknown trigger. An infectious agent has been suggested as one …

HTS-based diagnostics of sugarcane viruses: Seasonal variation and its implications for accurate detection

M Malapi-Wight, B Adhikari, J Zhou, L Hendrickson… - Viruses, 2021 - mdpi.com
Rapid global germplasm trade has increased concern about the spread of plant pathogens
and pests across borders that could become established, affecting agriculture and …