Current and emerging trends in techniques for plant pathogen detection

M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …

High‐throughput identification and diagnostics of pathogens and pests: overview and practical recommendations

L Tedersoo, R Drenkhan, S Anslan… - Molecular ecology …, 2019 - Wiley Online Library
High‐throughput identification technologies provide efficient tools for understanding the
ecology and functioning of microorganisms. Yet, these methods have been only rarely used …

Next generation sequencing for detection and discovery of plant viruses and viroids: comparison of two approaches

A Pecman, D Kutnjak, I Gutiérrez-Aguirre… - Frontiers in …, 2017 - frontiersin.org
Next generation sequencing (NGS) technologies are becoming routinely employed in
different fields of virus research. Different sequencing platforms and sample preparation …

Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description

B Lebas, I Adams, M Al Rwahnih, S Baeyen… - EPPO …, 2022 - Wiley Online Library
High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous
detection and potential identification of any organisms present in a sample. The growing …

Insect-specific viruses: from discovery to potential translational applications

S Nouri, EE Matsumura, YW Kuo, BW Falk - Current opinion in virology, 2018 - Elsevier
Highlights•NGS and bioinformatics analyses show that the insect virosphere contains many
previously undiscovered viruses of many taxa.•Insect-specific viruses (ISVs) do not infect …

Comparative study on three viral enrichment approaches based on RNA extraction for plant virus/viroid detection using high-throughput sequencing

YZA Gaafar, H Ziebell - PLoS One, 2020 - journals.plos.org
High-throughput sequencing (HTS) has become increasingly popular as virus diagnostic
tool. It has been used to detect and identify plant viruses and viroids in different types of …

High-throughput detection of a large set of viruses and viroids of pome and stone fruit trees by multiplex PCR-based amplicon sequencing

LC Costa, B Atha III, X Hu, K Lamour, Y Yang… - Frontiers in Plant …, 2022 - frontiersin.org
A comprehensive diagnostic method of known plant viruses and viroids is necessary to
provide an accurate phytosanitary status of fruit trees. However, most widely used detection …

Canine meningoencephalitis of unknown origin—the search for infectious agents in the cerebrospinal fluid via deep sequencing

JN Nessler, WK Jo, ADME Osterhaus… - Frontiers in Veterinary …, 2021 - frontiersin.org
Meningoencephalitis of unknown origin (MUO) describes a group of meningoencephalitides
in dogs with a hitherto unknown trigger. An infectious agent has been suggested as one …

Public domain databases: a gold mine for identification and genome reconstruction of plant viruses and viroids

VK Sidharthan, VK Baranwal - Genomics data analysis for crop …, 2024 - Springer
Plant viruses deprive the host due to their replication in the infected cells by hijacking the
host machinery and thereby affecting the quality and quantity of economic produce. In recent …

Looking beyond virus detection in RNA sequencing data: lessons learned from a community-based effort to detect cellular plant pathogens and pests

A Haegeman, Y Foucart, K De Jonghe, T Goedefroit… - Plants, 2023 - mdpi.com
High-throughput sequencing (HTS), more specifically RNA sequencing of plant tissues, has
become an indispensable tool for plant virologists to detect and identify plant viruses. During …