Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure
concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to …

Heterochromatin protein 1 (HP1) proteins were originally identified as critical components in
heterochromatin-mediated gene silencing and are now recognized to play essential roles in …

The formation of silenced and condensed heterochromatin foci involves enrichment of
heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid …

In mammals, HP1-mediated heterochromatin forms positionally and mechanically stable
genomic domains even though the component HP1 paralogs, HP1α, HP1β, and HP1γ …

The dynamic architecture of chromatin fibers, a key determinant of genome regulation, is
poorly understood. Here, we employ multimodal single-molecule Förster resonance energy …

DNA is stored in vivo in a highly compact, so-called condensed phase, where gene
regulatory processes are governed by the intricate interplay between different states of DNA …

Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining
diffusion and interaction/binding properties in biological and material sciences. An …

The binding of nuclear proteins to chromatin in live cells has been analyzed by kinetic
modeling procedures applied to experimental data from fluorescence recovery after …

To understand the function of cellular protein networks, spatial and temporal context is
essential. Fluorescence correlation spectroscopy (FCS) is a single-molecule method to …

Diffusion processes and local dynamic equilibria inside cells lead to nonuniform spatial
distributions of molecules, which are essential for processes such as nuclear organization …