The de novo synthesis of genomes has made unprecedented progress and achieved
milestones, particularly in bacteria and yeast. However, the process of synthesizing a …

Careful consideration of how we approach design is crucial to all areas of biotechnology.
However, choosing or develo** an effective design methodology is not always easy as …

Synthetic genomics seeks to design and construct entire genomes to mechanistically dissect
fundamental questions of genome function and to engineer organisms for diverse …

Biosynthesis is the application of enzymes in microbial cell factories and has emerged as a
promising alternative to chemical synthesis. However, natural enzymes with limited catalytic …

In the era of synthetic biology, design, construction, and utilization of synthetic chromosomes
with unique features provide a strategy to study complex cellular processes such as aging …

Chromosome engineering is a transformative field at the cutting edge of biological science,
offering unprecedented precision in manipulating large-scale genomic DNA within cells …

After decades of development, DNA synthesis, assembly, and sequencing technologies
have reached a high level, allowing faster and cheaper acquirements of synthetic genes or …

Microbes have long provided us with important capabilities, and the genome engineering of
microbes has greatly empowered research and applications in biotechnology. This is …

We designed and synthesized synI, which is∼ 21.6% shorter than native chrI, the smallest
chromosome in Saccharomyces cerevisiae. SynI was designed for attachment to another …

With the rapid advance in synthetic biology and the expanding field of synthetic genomics,
the realization of a redesigned yeast genome has become an achievable milestone. Multiple …