Sample preparation is a major task in a regulated bioanalytical laboratory. The sample
preparation procedure significantly impacts assay throughput, data quality, analysis cost …

Substitution of acetonitrile (ACN) as organic modifier in mobile phases for liquid
chromatography by mixtures of propylene carbonate (PC) and ethanol (EtOH) may be …

Comprehensive two-dimensional liquid chromatography (LC× LC) is an attractive separation
technique that allows achieving high peak capacities and information on chemical …

Phase ratio Φ for an HPLC column is a common parameter being defined as the ratio
between the volume of the stationary phase V st and the void volume of the column V 0 …

For a chromatographic column, phase ratio Φ is defined as the ratio between the volume of
the stationary phase V st and the void volume of the column V 0, and it is an important …

Green bioanalytical approaches are oriented toward minimization or elimination of
hazardous chemicals associated to bioanalytical applications. LC/MS–MS assay of enalapril …

Nitrosamines are carcinogens substances firstly detected in sartans drugs in 2018, leading
to new regulations and monitoring programmes that raised the costs and challenges to the …

Limonene, considered a green solvent, was successfully used to extract simvastatin,
lovastatin, and their hydroxy‐acid metabolites from human plasma samples. The extraction …

A new perspective in the lipophilicity evaluation through RP-HPLC is permitted by analysis
of the retention factor (k) obtained by injecting large volumes of test samples prepared in …

Recent studies showed that injection of large volume of hydrophobic solvents used as
sample diluents could be applied in reversed-phase liquid chromatography (RP-LC). This …