[HTML][HTML] Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …
9) shows the opportunity to treat a diverse array of untreated various genetic and …
CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects
H Manghwar, B Li, X Ding, A Hussain… - Advanced …, 2020 - Wiley Online Library
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
Human genetic diversity alters off-target outcomes of therapeutic gene editing
CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat
disease. However, standard computational and biochemical methods to predict off-target …
disease. However, standard computational and biochemical methods to predict off-target …
A new age of precision gene therapy
A Schambach, CJ Buchholz, R Torres-Ruiz, K Cichutek… - The Lancet, 2024 - thelancet.com
Gene therapy has become a clinical reality as market-approved advanced therapy medicinal
products for the treatment of distinct monogenetic diseases and B-cell malignancies. This …
products for the treatment of distinct monogenetic diseases and B-cell malignancies. This …
In vivo CRISPR editing with no detectable genome-wide off-target mutations
P Akcakaya, ML Bobbin, JA Guo, J Malagon-Lopez… - Nature, 2018 - nature.com
CRISPR–Cas genome-editing nucleases hold substantial promise for develo** human
therapeutic applications,,,,–but identifying unwanted off-target mutations is important for …
therapeutic applications,,,,–but identifying unwanted off-target mutations is important for …
CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
Current methods can illuminate the genome-wide activity of CRISPR–Cas9 nucleases, but
are not easily scalable to the throughput needed to fully understand the principles that …
are not easily scalable to the throughput needed to fully understand the principles that …
Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9‐edited cotton plants
J Li, H Manghwar, L Sun, P Wang… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas9 system has been extensively applied for crop improvement. However,
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …
Evaluating and enhancing target specificity of gene-editing nucleases and deaminases
Programmable nucleases and deaminases, which include zinc-finger nucleases,
transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA …
transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA …
Genome-wide detection of CRISPR editing in vivo using GUIDE-tag
Abstract Analysis of off-target editing is an important aspect of the development of safe
nuclease-based genome editing therapeutics. in vivo assessment of nuclease off-target …
nuclease-based genome editing therapeutics. in vivo assessment of nuclease off-target …
The current state and future of CRISPR-Cas9 gRNA design tools
Recent years have seen the development of computational tools to assist researchers in
performing CRISPR-Cas9 experiment optimally. More specifically, these tools aim to …
performing CRISPR-Cas9 experiment optimally. More specifically, these tools aim to …