Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP
Single-cell proteomics by mass spectrometry (MS) allows the quantification of proteins with
high specificity and sensitivity. To increase its throughput, we developed nano-proteomic …
high specificity and sensitivity. To increase its throughput, we developed nano-proteomic …
A tutorial review of labeling methods in mass spectrometry-based quantitative proteomics
Recent advancements in mass spectrometry (MS) have revolutionized quantitative
proteomics, with multiplex isotope labeling emerging as a key strategy for enhancing …
proteomics, with multiplex isotope labeling emerging as a key strategy for enhancing …
Easy and accessible workflow for label-free single-cell proteomics
Single-cell proteomics (SCP) can provide information that is unattainable through either bulk-
scale protein measurements or single-cell profiling of other omes. Maximizing proteome …
scale protein measurements or single-cell profiling of other omes. Maximizing proteome …
Micropillar arrays, wide window acquisition and AI-based data analysis improve comprehensiveness in multiple proteomic applications
M Matzinger, A Schmücker, R Yelagandula… - Nature …, 2024 - nature.com
Comprehensive proteomic analysis is essential to elucidate molecular pathways and protein
functions. Despite tremendous progress in proteomics, current studies still suffer from limited …
functions. Despite tremendous progress in proteomics, current studies still suffer from limited …
What's new in single-cell proteomics
Highlights•Recent advances in single-cell proteomics.•Improvements in sample preparation,
separation techniques, mass spectrometry instrumentation, and data acquisition/analysis.• …
separation techniques, mass spectrometry instrumentation, and data acquisition/analysis.• …
Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry
The rapid evolution of mass spectrometry-based single-cell proteomics now enables the
cataloging of several thousand proteins from single cells. We investigated whether we could …
cataloging of several thousand proteins from single cells. We investigated whether we could …
Boosting the Sensitivity of Quantitative Single-Cell Proteomics with Infrared-Tandem Mass Tags
Single-cell proteomics is a powerful approach to precisely profile protein landscapes within
individual cells toward a comprehensive understanding of proteomic functions and tissue …
individual cells toward a comprehensive understanding of proteomic functions and tissue …
Increasing the throughput and reproducibility of activity-based proteome profiling studies with hyperplexing and intelligent data acquisition
HG Budayeva, TP Ma, S Wang, M Choi… - Journal of Proteome …, 2024 - ACS Publications
Intelligent data acquisition (IDA) strategies, such as a real-time database search (RTS), have
improved the depth of proteome coverage for experiments that utilize isobaric labels and …
improved the depth of proteome coverage for experiments that utilize isobaric labels and …
Are we there yet? Assessing the readiness of single-cell proteomics to answer biological hypotheses
AA Nitz, JH Giraldez Chavez, ZG Eliason… - Journal of Proteome …, 2024 - ACS Publications
Single-cell analysis is an active area of research in many fields of biology. Measurements at
single-cell resolution allow researchers to study diverse populations without losing …
single-cell resolution allow researchers to study diverse populations without losing …
Toward Single Bacterium Proteomics
Bacteria are orders of magnitude smaller than mammalian cells, and while single cell
proteomics (SCP) currently detects and quantifies several thousands of proteins per …
proteomics (SCP) currently detects and quantifies several thousands of proteins per …