Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique

J Valli, A Garcia-Burgos, LM Rooney… - Journal of Biological …, 2021 - ASBMB
Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Rational design of true monomeric and bright photoactivatable fluorescent proteins

M Zhang, H Chang, Y Zhang, J Yu, L Wu, W Ji… - Nature …, 2012 - nature.com
Monomeric (m) Eos2 is an engineered photoactivatable fluorescent protein widely used for
super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations …

Photoinduced chemistry in fluorescent proteins: curse or blessing?

A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Fluorescent proteins for live-cell imaging with super-resolution

K Nienhaus, GU Nienhaus - Chemical Society Reviews, 2014 - pubs.rsc.org
Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

Single cell optical imaging and spectroscopy

AS Stender, K Marchuk, C Liu, S Sander… - Chemical …, 2013 - ACS Publications
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …

Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate

N Durisic, L Laparra-Cuervo, Á Sandoval-Álvarez… - Nature …, 2014 - nature.com
Photoswitchable fluorescent probes are central to localization-based super-resolution
microscopy. Among these probes, fluorescent proteins are appealing because they are …

Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions

O Rossier, V Octeau, JB Sibarita, C Leduc… - Nature cell …, 2012 - nature.com
Integrins in focal adhesions (FAs) mediate adhesion and force transmission to extracellular
matrices essential for cell motility, proliferation and differentiation. Different fibronectin …

Quantitative photo activated localization microscopy: unraveling the effects of photoblinking

P Annibale, S Vanni, M Scarselli, U Rothlisberger… - PloS one, 2011 - journals.plos.org
In this work we discuss how to use photophysical information for improved quantitative
measurements using Photo Activated Localization Microscopy (PALM) imaging. We …

Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting

SL Veatch, BB Machta, SA Shelby, EN Chiang… - PloS one, 2012 - journals.plos.org
We present an analytical method using correlation functions to quantify clustering in super-
resolution fluorescence localization images and electron microscopy images of static …