Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique
J Valli, A Garcia-Burgos, LM Rooney… - Journal of Biological …, 2021 - ASBMB
Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …
life sciences to study minute cellular structures and processes. However, with the increasing …
Rational design of true monomeric and bright photoactivatable fluorescent proteins
Monomeric (m) Eos2 is an engineered photoactivatable fluorescent protein widely used for
super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations …
super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations …
Photoinduced chemistry in fluorescent proteins: curse or blessing?
A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …
Fluorescent proteins for live-cell imaging with super-resolution
K Nienhaus, GU Nienhaus - Chemical Society Reviews, 2014 - pubs.rsc.org
Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …
Single cell optical imaging and spectroscopy
AS Stender, K Marchuk, C Liu, S Sander… - Chemical …, 2013 - ACS Publications
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …
made using a microscope, including compartment-like structures in cork samples that he …
Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate
N Durisic, L Laparra-Cuervo, Á Sandoval-Álvarez… - Nature …, 2014 - nature.com
Photoswitchable fluorescent probes are central to localization-based super-resolution
microscopy. Among these probes, fluorescent proteins are appealing because they are …
microscopy. Among these probes, fluorescent proteins are appealing because they are …
Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions
Integrins in focal adhesions (FAs) mediate adhesion and force transmission to extracellular
matrices essential for cell motility, proliferation and differentiation. Different fibronectin …
matrices essential for cell motility, proliferation and differentiation. Different fibronectin …
Quantitative photo activated localization microscopy: unraveling the effects of photoblinking
In this work we discuss how to use photophysical information for improved quantitative
measurements using Photo Activated Localization Microscopy (PALM) imaging. We …
measurements using Photo Activated Localization Microscopy (PALM) imaging. We …
Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting
We present an analytical method using correlation functions to quantify clustering in super-
resolution fluorescence localization images and electron microscopy images of static …
resolution fluorescence localization images and electron microscopy images of static …