Process analytical technique (PAT) miniaturization for monoclonal antibody aggregate detection in continuous downstream processing

MN São Pedro, ME Klijn, MHM Eppink… - Journal of Chemical …, 2022‏ - Wiley Online Library
The transition to continuous biomanufacturing is considered the next step to reduce costs
and improve process robustness in the biopharmaceutical industry, while also improving …

Field-flow fractionation: New and exciting perspectives in polymer analysis

MI Malik, H Pasch - Progress in Polymer Science, 2016‏ - Elsevier
The development of advanced polymeric materials requires state-of-the-art synthesis and
molecular characterization protocols. Only the precise knowledge of molecular structure …

Role of carbonyl modifications on aging-associated protein aggregation

M Tanase, AM Urbanska, V Zolla, CC Clement… - Scientific reports, 2016‏ - nature.com
Protein aggregation is a common biological phenomenon, observed in different
physiological and pathological conditions. Decreased protein solubility and a tendency to …

The dual nature of biomimetic melanin

A Mavridi-Printezi, S Giordani, A Menichetti, D Mordini… - Nanoscale, 2024‏ - pubs.rsc.org
Melanin-inspired nanomaterials offer unique photophysical, electronic and radical
scavenging properties that are widely explored for health and environmental preservation …

Tracking heme-protein interactions in healthy and pathological human serum in native conditions by miniaturized FFF-multidetection

V Marassi, S Giordani, P Reschiglian, B Roda… - Applied Sciences, 2022‏ - mdpi.com
The interaction of heme with blood serum proteins plays an important role in many
physiological and pathological processes involving enzyme activity, gene expression and …

Field-flow fractionation: A gentle separation and characterization technique in biomedicine

X Zhang, Y Li, S Shen, S Lee, H Dou - TrAC Trends in Analytical Chemistry, 2018‏ - Elsevier
Abstract Field-flow fractionation (FFF), a gentle tool for separation and characterization of
macromolecules and particles, has attracted increasing interest in recent years due to its …

Field-flow fractionation in molecular biology and biotechnology

S Giordani, V Marassi, A Placci, A Zattoni, B Roda… - Molecules, 2023‏ - mdpi.com
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to
gently separate and characterize nano-and microsystems in suspension. These techniques …

An ultracentrifugation–hollow-fiber flow field-flow fractionation orthogonal approach for the purification and map** of extracellular vesicle subtypes

V Marassi, S Maggio, M Battistelli, V Stocchi… - … of Chromatography A, 2021‏ - Elsevier
In the course of their life span, cells release a multitude of different vesicles in the
extracellular matrix (EVs), constitutively and/or upon stimulation, carrying signals either …

Characterization of red wine native colloids by asymmetrical flow field-flow fractionation with online multidetection

V Marassi, M Marangon, A Zattoni, S Vincenzi… - Food …, 2021‏ - Elsevier
Colloids are an important component of wines, but their study is challenging due to their
instability. Asymmetrical Flow-Field Flow Fractionation (AF4)-multidetection is here …

Application of af4-multidetection to liraglutide in its formulation: Preserving and representing native aggregation

V Marassi, M Macis, S Giordani, L Ferrazzano… - Molecules, 2022‏ - mdpi.com
Aggregation is among the most critical parameters affecting the pharmacological and safety
profile of peptide Active Pharmaceutical Ingredients (APIs). For this reason, it is of utmost …