The molecular basis for cellular function of intrinsically disordered protein regions

AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024‏ - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …

A guide to membraneless organelles and their various roles in gene regulation

T Hirose, K Ninomiya, S Nakagawa… - … Reviews Molecular Cell …, 2023‏ - nature.com
Membraneless organelles (MLOs) are detected in cells as dots of mesoscopic size. By
undergoing phase separation into a liquid-like or gel-like phase, MLOs contribute to …

[HTML][HTML] Functional partitioning of transcriptional regulators by patterned charge blocks

H Lyons, RT Veettil, P Pradhan, C Fornero… - Cell, 2023‏ - cell.com
Components of transcriptional machinery are selectively partitioned into specific
condensates, often mediated by protein disorder, yet we know little about how this specificity …

Modulating biomolecular condensates: a novel approach to drug discovery

DM Mitrea, M Mittasch, BF Gomes, IA Klein… - Nature Reviews Drug …, 2022‏ - nature.com
In the past decade, membraneless assemblies known as biomolecular condensates have
been reported to play key roles in many cellular functions by compartmentalizing specific …

Capillary forces generated by biomolecular condensates

B Gouveia, Y Kim, JW Shaevitz, S Petry, HA Stone… - Nature, 2022‏ - nature.com
Liquid–liquid phase separation and related phase transitions have emerged as generic
mechanisms in living cells for the formation of membraneless compartments or biomolecular …

Molecular crowding: the history and development of a scientific paradigm

C Alfano, Y Fichou, K Huber, M Weiss, E Spruijt… - Chemical …, 2024‏ - ACS Publications
It is now generally accepted that macromolecules do not act in isolation but “live” in a
crowded environment, that is, an environment populated by numerous different molecules …

Liquid–liquid phase separation in human health and diseases

B Wang, L Zhang, T Dai, Z Qin, H Lu, L Zhang… - Signal transduction and …, 2021‏ - nature.com
Emerging evidence suggests that liquid–liquid phase separation (LLPS) represents a vital
and ubiquitous phenomenon underlying the formation of membraneless organelles in …

Condensates formed by prion-like low-complexity domains have small-world network structures and interfaces defined by expanded conformations

M Farag, SR Cohen, WM Borcherds, A Bremer… - Nature …, 2022‏ - nature.com
Biomolecular condensates form via coupled associative and segregative phase transitions
of multivalent associative macromolecules. Phase separation coupled to percolation is one …

Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu

M Poudyal, K Patel, L Gadhe, AS Sawner… - Nature …, 2023‏ - nature.com
Liquid-liquid phase separation (LLPS) has emerged as a crucial biological phenomenon
underlying the sequestration of macromolecules (such as proteins and nucleic acids) into …

Aberrant phase separation and nucleolar dysfunction in rare genetic diseases

MA Mensah, H Niskanen, AP Magalhaes, S Basu… - Nature, 2023‏ - nature.com
Thousands of genetic variants in protein-coding genes have been linked to disease.
However, the functional impact of most variants is unknown as they occur within intrinsically …