Best practices in metabarcoding of fungi: from experimental design to results
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …
Mycobiome diversity: high-throughput sequencing and identification of fungi
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
Primer, pipelines, parameters: issues in 16S rRNA gene sequencing
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …
investigating microbiomes. However, comparative studies on differences in procedures are …
Revisiting plant–microbe interactions and microbial consortia application for enhancing sustainable agriculture: a review
The present scenario of agricultural sector is dependent hugely on the use of chemical-
based fertilizers and pesticides that impact the nutritional quality, health status, and …
based fertilizers and pesticides that impact the nutritional quality, health status, and …
Benchmarking metagenomics tools for taxonomic classification
Metagenomic sequencing is revolutionizing the detection and characterization of microbial
species, and a wide variety of software tools are available to perform taxonomic …
species, and a wide variety of software tools are available to perform taxonomic …
Shotgun metagenomics, from sampling to analysis
Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes
have crucial roles in the environment and in human health. However, microbes are …
have crucial roles in the environment and in human health. However, microbes are …
Consistent and correctable bias in metagenomic sequencing experiments
Marker-gene and metagenomic sequencing have profoundly expanded our ability to
measure biological communities. But the measurements they provide differ from the truth …
measure biological communities. But the measurements they provide differ from the truth …
Identifying biases and their potential solutions in human microbiome studies
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …
The madness of microbiome: attempting to find consensus “best practice” for 16S microbiome studies
The development and continuous improvement of high-throughput sequencing platforms
have stimulated interest in the study of complex microbial communities. Currently, the most …
have stimulated interest in the study of complex microbial communities. Currently, the most …
A review of methods and databases for metagenomic classification and assembly
Microbiome research has grown rapidly over the past decade, with a proliferation of new
methods that seek to make sense of large, complex data sets. Here, we survey two of the …
methods that seek to make sense of large, complex data sets. Here, we survey two of the …