The molecular basis for cellular function of intrinsically disordered protein regions

AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …

Classification of intrinsically disordered regions and proteins

R Van Der Lee, M Buljan, B Lang, RJ Weatheritt… - Chemical …, 2014 - ACS Publications
Over the past decade, we have observed a massive increase in the amount of information
describing protein sequences from a variety of organisms. 1, 2 While this may reflect the …

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1

DJ Klionsky, AK Abdel-Aziz, S Abdelfatah, M Abdellatif… - autophagy, 2021 - Taylor & Francis
In 2008, we published the first set of guidelines for standardizing research in autophagy.
Since then, this topic has received increasing attention, and many scientists have entered …

Molecular interactions underlying liquid− liquid phase separation of the FUS low-complexity domain

AC Murthy, GL Dignon, Y Kan, GH Zerze… - Nature structural & …, 2019 - nature.com
The low-complexity domain of the RNA-binding protein FUS (FUS LC) mediates liquid−
liquid phase separation (LLPS), but the interactions between the repetitive SYGQ-rich …

Intrinsically unstructured proteins and their functions

HJ Dyson, PE Wright - Nature reviews Molecular cell biology, 2005 - nature.com
Many gene sequences in eukaryotic genomes encode entire proteins or large segments of
proteins that lack a well-structured three-dimensional fold. Disordered regions can be highly …

Intrinsically disordered proteins and their “mysterious”(meta) physics

VN Uversky - Frontiers in Physics, 2019 - frontiersin.org
Recognition of the natural abundance and functional importance of intrinsically disordered
proteins (IDPs), and protein hybrids that contain both intrinsically disordered protein regions …

Widespread occurrence of the droplet state of proteins in the human proteome

M Hardenberg, A Horvath, V Ambrus… - Proceedings of the …, 2020 - National Acad Sciences
A wide range of proteins have been reported to condensate into a dense liquid phase,
forming a reversible droplet state. Failure in the control of the droplet state can lead to the …

IUPred: web server for the prediction of intrinsically unstructured regions of proteins based on estimated energy content

Z Dosztányi, V Csizmok, P Tompa, I Simon - Bioinformatics, 2005 - academic.oup.com
Intrinsically unstructured/disordered proteins and domains (IUPs) lack a well-defined three-
dimensional structure under native conditions. The IUPred server presents a novel algorithm …

[HTML][HTML] A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins

AG Kikhney, DI Svergun - FEBS letters, 2015 - Elsevier
Small-angle X-ray scattering (SAXS) is a biophysical method to study the overall shape and
structural transitions of biological macromolecules in solution. SAXS provides low resolution …

Intrinsically Disordered Proteins in Human Diseases: Introducing the D2 Concept

VN Uversky, CJ Oldfield, AK Dunker - Annu. Rev. Biophys., 2008 - annualreviews.org
Intrinsically disordered proteins (IDPs) lack stable tertiary and/or secondary structures under
physiological conditions in vitro. They are highly abundant in nature and their functional …