A high-throughput multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors

D Koveal, PC Rosen, DJ Meyer… - Nature …, 2022 - nature.com
Genetically encoded fluorescent biosensors are powerful tools used to track chemical
processes in intact biological systems. However, the development and optimization of …

Photophysical engineering of fluorescent proteins: Accomplishments and challenges of physical chemistry strategies

S Mukherjee, R Jimenez - The Journal of Physical Chemistry B, 2022 - ACS Publications
Fluorescent proteins (FPs) have become ubiquitous tools for biological research and
concomitantly they are intriguing molecules that are amenable to study with a wide range of …

Automating the high-throughput screening of protein-based optical indicators and actuators

J Lee, B Campillo, S Hamidian, Z Liu, M Shorey… - Biochemistry, 2022 - ACS Publications
Over the last 25 years, protein engineers have developed an impressive collection of optical
tools to interface with biological systems: indicators to eavesdrop on cellular activity and …

Multiphoton bleaching of red fluorescent proteins and the ways to reduce it

M Drobizhev, RS Molina, J Franklin - International journal of molecular …, 2022 - mdpi.com
Red fluorescent proteins and biosensors built upon them are potentially beneficial for two-
photon laser microscopy (TPLM) because they can image deeper layers of tissue, compared …

Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe

Y Sun, B Wu, X Liu, L Liu, S Zhou, Y Feng - Molecules, 2024 - mdpi.com
This article reports three new two-photon absorption (TPA) materials that are quinolinium-
carbazole derivates. They are 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (M4) …