Yeast expression systems: overview and recent advances
Yeasts are outstanding hosts for the production of functional recombinant proteins with
industrial or medical applications. Great attention has been emerged on yeast due to the …
industrial or medical applications. Great attention has been emerged on yeast due to the …
[HTML][HTML] Metabolic engineering of Pichia pastoris
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …
[HTML][HTML] Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris
A Weninger, AM Hatzl, C Schmid, T Vogl… - Journal of …, 2016 - Elsevier
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is one of the most commonly
used expression systems for heterologous protein production. However the recombination …
used expression systems for heterologous protein production. However the recombination …
An updated view on horseradish peroxidases: recombinant production and biotechnological applications
FW Krainer, A Glieder - Applied microbiology and biotechnology, 2015 - Springer
Horseradish peroxidase has been the subject of scientific research for centuries. It has been
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …
An affinity-enhanced, broadly neutralizing heavy chain–only antibody protects against SARS-CoV-2 infection in animal models
Broadly neutralizing antibodies are an important treatment for individuals with coronavirus
disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 …
disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 …
Synthetic biology and molecular genetics in non-conventional yeasts: current tools and future advances
Coupling the tools of synthetic biology with traditional molecular genetic techniques can
enable the rapid prototy** and optimization of yeast strains. While the era of yeast …
enable the rapid prototy** and optimization of yeast strains. While the era of yeast …
[HTML][HTML] Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects
H Raschmanová, A Weninger, A Glieder, K Kovar… - Biotechnology …, 2018 - Elsevier
Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …
engineering, while also changing the speed and efficiency of metabolic engineering in …
Towards systems metabolic engineering in Pichia pastoris
JP Schwarzhans, T Luttermann, M Geier… - Biotechnology …, 2017 - Elsevier
The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …
Microbial lignin peroxidases: applications, production challenges and future perspectives
Lignin serves as the most abundant source of aromatic high-value products, but it has
remained underexploited due to its inert and recalcitrant nature. White-rot basidiomycetes …
remained underexploited due to its inert and recalcitrant nature. White-rot basidiomycetes …
Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers
A Weninger, JE Fischer… - Journal of cellular …, 2018 - Wiley Online Library
Komagataella phaffii (syn. Pichia pastoris) is one of the most commonly used host systems
for recombinant protein expression. Achieving targeted genetic modifications had been …
for recombinant protein expression. Achieving targeted genetic modifications had been …