One hundred years of clostridial butanol fermentation

HG Moon, YS Jang, C Cho, J Lee… - FEMS microbiology …, 2016 - academic.oup.com
Butanol has been widely used as an important industrial solvent and feedstock for chemical
production. Also, its superior fuel properties compared with ethanol make butanol a good …

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

S Li, L Huang, C Ke, Z Pang, L Liu - Biotechnology for biofuels, 2020 - Springer
The global energy crisis and limited supply of petroleum fuels have rekindled the interest in
utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a …

Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

J Zhang, W Zong, W Hong, ZT Zhang, Y Wang - Metabolic engineering, 2018 - Elsevier
Abstract Although CRISPR-Cas9/Cpf1 have been employed as powerful genome
engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into …

Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii …

Y Wang, ZT Zhang, SO Seo, P Lynn, T Lu… - ACS synthetic …, 2016 - ACS Publications
CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …

Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system

Y Wang, ZT Zhang, SO Seo, K Choi, T Lu, YS **… - Journal of …, 2015 - Elsevier
The anaerobic spore-forming, gram-positive, solventogenic clostridia are notorious for being
difficult to genetically engineer. Based on CRISPR/Cas9 assisted homologous …

Biobutanol production from sugarcane straw: Defining optimal biomass loading for improved ABE fermentation

B Pratto, V Chandgude, R de Sousa Junior… - Industrial crops and …, 2020 - Elsevier
Key objective of this work was to evaluate the use of cellulosic fraction from sugarcane straw
pretreated by liquid hot water (LHW) for butanol production via acetone-butanol-ethanol …

Towards comprehensive lignocellulosic biomass utilization for bioenergy production: Efficient biobutanol production from acetic acid pretreated switchgrass with …

P Wang, YM Chen, Y Wang, YY Lee, W Zong, S Taylor… - Applied Energy, 2019 - Elsevier
For bioenergy production from lignocellulosic biomass, most biomass pretreatment
processes need to use some chemical reagent as the catalyst to overcome the biomass …

Genome editing in Clostridium saccharoperbutylacetonicum N1-4 with the CRISPR-Cas9 system

S Wang, S Dong, P Wang, Y Tao… - Applied and …, 2017 - Am Soc Microbiol
Clostridium saccharoperbutylacetonicum N1-4 is well known as a hyper-butanol-producing
strain. However, the lack of genetic engineering tools hinders further elucidation of its …

Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis

PJ Enyeart, G Mohr, AD Ellington, AM Lambowitz - Mobile DNA, 2014 - Springer
Mobile group II introns are bacterial retrotransposons that combine the activities of an
autocatalytic intron RNA (a ribozyme) and an intron-encoded reverse transcriptase to insert …

[HTML][HTML] Clostridium novyi-NT in cancer therapy

V Staedtke, NJ Roberts, RY Bai, S Zhou - Genes & diseases, 2016 - Elsevier
The attenuated anaerobic bacterium Clostridium novyi-NT (C. novyi-NT) is known for its
ability to precisely germinate in and eradicate treatment-resistant hypoxic tumors in various …