The ability to express or deplete proteins in living cells is crucial for the study of biological
processes. Viral vectors are often useful to deliver DNA constructs to cells that are difficult to …

Intracellular delivery, the process of transporting substances into cells, is crucial for various
applications, such as drug delivery, gene therapy, cell imaging, and regenerative medicine …

A major reason for the current reproducibility crisis in the life sciences is the poor
implementation of quality control measures and reporting standards. Improvement is …

Background Constitutive promoters that ensure sustained and high level gene expression
are basic research tools that have a wide range of applications, including studies of human …

In recent years, great breakthroughs have been made in basic research and clinical
applications of stem cells in regenerative medicine and other fields, which continue to …

Since the first reports on isolation of pluripotent mouse embryonic stem (ES) cells 3 decades
ago, there have been numerous attempts to derive ES cell lines from commercially important …

Human induced pluripotent stem cells (hiPSC) hold promise for regenerative therapies,
though there are several safety concerns including the risk of oncogenic transformation or …

This paper presents the development of an automated microinjection system with high
productivity for small cells. Compared with many existing microinjection systems that are …

To enable robust expression of transgenes in stem cells, recombinase-mediated cassette
exchange at safe harbor loci is frequently adopted. The choice of recombinase enzyme is a …

Neurokinin B (NKB), a member of the tachykinin (TAC) family, plays important roles in
mammalian neuropeptide secretion in related to reproduction. However, its potential role in …