Recombinant protein expression in Escherichia coli: advances and challenges
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …
Its use as a cell factory is well-established and it has become the most popular expression …
Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins
One of the most important branches of genetic engineering is the expression of recombinant
proteins using biological expression systems. Nowadays, different expression systems are …
proteins using biological expression systems. Nowadays, different expression systems are …
Lipases for biotechnology
KE Jaeger, T Eggert - Current opinion in biotechnology, 2002 - Elsevier
Lipases constitute the most important group of biocatalysts for biotechnological applications.
The high-level production of microbial lipases requires not only the efficient overexpression …
The high-level production of microbial lipases requires not only the efficient overexpression …
scFv antibody: principles and clinical application
To date, generation of single‐chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen‐binding fragment in bacterial …
technique used to produce a completely functional antigen‐binding fragment in bacterial …
[BOOK][B] Principles of protein X-ray crystallography
J Drenth - 2007 - books.google.com
X-ray crystallography is an established method for studying the structure of proteins and
other macromolecules. As the importance of proteins grows, researchers in many fields have …
other macromolecules. As the importance of proteins grows, researchers in many fields have …
Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems
K Terpe - Applied microbiology and biotechnology, 2006 - Springer
During the proteomics period, the growth in the use of recombinant proteins has increased
greatly in the recent years. Bacterial systems remain most attractive due to low cost, high …
greatly in the recent years. Bacterial systems remain most attractive due to low cost, high …
Advanced genetic strategies for recombinant protein expression in Escherichia coli
HP Sørensen, KK Mortensen - Journal of biotechnology, 2005 - Elsevier
Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …
Hence, recombinant protein production is frequently the sole applicable procedure. The …
Structural basis for molecular recognition of folic acid by folate receptors
Folate receptors (FRα, FRβ and FRγ) are cysteine-rich cell-surface glycoproteins that bind
folate with high affinity to mediate cellular uptake of folate. Although expressed at very low …
folate with high affinity to mediate cellular uptake of folate. Although expressed at very low …
Solubilization and refolding of bacterial inclusion body proteins
SM Singh, AK Panda - Journal of bioscience and bioengineering, 2005 - Elsevier
Inclusion bodies produced in Escherichia coli are composed of densely packed denatured
protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive …
protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive …
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein
Existing protein tagging and detection methods are powerful but have drawbacks. Split
protein tags can perturb protein solubility,,, or may not work in living cells,,. Green …
protein tags can perturb protein solubility,,, or may not work in living cells,,. Green …