Recombinant protein expression in Escherichia coli: advances and challenges

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

M Karbalaei, SA Rezaee… - Journal of cellular …, 2020 - Wiley Online Library
One of the most important branches of genetic engineering is the expression of recombinant
proteins using biological expression systems. Nowadays, different expression systems are …

Lipases for biotechnology

KE Jaeger, T Eggert - Current opinion in biotechnology, 2002 - Elsevier
Lipases constitute the most important group of biocatalysts for biotechnological applications.
The high-level production of microbial lipases requires not only the efficient overexpression …

scFv antibody: principles and clinical application

ZA Ahmad, SK Yeap, AM Ali, WY Ho… - Journal of …, 2012 - Wiley Online Library
To date, generation of single‐chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen‐binding fragment in bacterial …

[BOOK][B] Principles of protein X-ray crystallography

J Drenth - 2007 - books.google.com
X-ray crystallography is an established method for studying the structure of proteins and
other macromolecules. As the importance of proteins grows, researchers in many fields have …

Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems

K Terpe - Applied microbiology and biotechnology, 2006 - Springer
During the proteomics period, the growth in the use of recombinant proteins has increased
greatly in the recent years. Bacterial systems remain most attractive due to low cost, high …

Advanced genetic strategies for recombinant protein expression in Escherichia coli

HP Sørensen, KK Mortensen - Journal of biotechnology, 2005 - Elsevier
Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …

Structural basis for molecular recognition of folic acid by folate receptors

C Chen, J Ke, XE Zhou, W Yi, JS Brunzelle, J Li… - Nature, 2013 - nature.com
Folate receptors (FRα, FRβ and FRγ) are cysteine-rich cell-surface glycoproteins that bind
folate with high affinity to mediate cellular uptake of folate. Although expressed at very low …

Solubilization and refolding of bacterial inclusion body proteins

SM Singh, AK Panda - Journal of bioscience and bioengineering, 2005 - Elsevier
Inclusion bodies produced in Escherichia coli are composed of densely packed denatured
protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive …

Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein

S Cabantous, TC Terwilliger, GS Waldo - Nature biotechnology, 2005 - nature.com
Existing protein tagging and detection methods are powerful but have drawbacks. Split
protein tags can perturb protein solubility,,, or may not work in living cells,,. Green …