To flourish, cancers greatly depend on their surrounding tumor microenvironment (TME),
and cancer-associated fibroblasts (CAFs) in TME are critical for cancer occurrence and …

Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

Genome editing holds great potential for cancer treatment due to the ability to precisely
inactivate or repair cancer-related genes. However, delivery of CRISPR/Cas to solid tumours …

One of the problems that has slowed the development and approval of new anticancer
therapies is the lack of preclinical models that can be used to identify key molecular, cellular …

Three‐dimensional cell culture models, such as spheroids, can be used in the process of the
development of new anticancer agents because they are able to closely mimic the main …

Innovations in biomaterials and stem cell technology have allowed for the emergence of
novel three-dimensional (3D) tissue-like structures known as organoids and spheroids. As a …

The cell microenvironment has emerged as a key determinant of cell behavior and function
in development, physiology, and pathophysiology. The extracellular matrix (ECM) within the …

This paper discusses “bioink”, bioprintable materials used in three dimensional (3D)
bioprinting processes, where cells and other biologics are deposited in a spatially controlled …

The past decades have witnessed significant efforts toward the development of three-
dimensional (3D) cell cultures as systems that better mimic in vivo physiology. Today, 3D …

In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some
features of solid tumors, such as their spatial architecture, physiological responses …