Recent advances in CRISPR-based genome editing technology and its applications in cardiovascular research
ZH Li, J Wang, JP Xu, J Wang, X Yang - Military Medical Research, 2023 - Springer
The rapid development of genome editing technology has brought major breakthroughs in
the fields of life science and medicine. In recent years, the clustered regularly interspaced …
the fields of life science and medicine. In recent years, the clustered regularly interspaced …
Tools for experimental and computational analyses of off-target editing by programmable nucleases
Genome editing using programmable nucleases is revolutionizing life science and
medicine. Off-target editing by these nucleases remains a considerable concern, especially …
medicine. Off-target editing by these nucleases remains a considerable concern, especially …
[HTML][HTML] R-loop formation and conformational activation mechanisms of Cas9
Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA
cleavage,–. The programmable activity of Cas9 has been widely utilized for genome editing …
cleavage,–. The programmable activity of Cas9 has been widely utilized for genome editing …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits
relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered …
relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered …
Cas12a domain flexibility guides R-loop formation and forces RuvC resetting
The specific nature of CRISPR-Cas12a makes it a desirable RNA-guided endonuclease for
biotechnology and therapeutic applications. To understand how R-loop formation within the …
biotechnology and therapeutic applications. To understand how R-loop formation within the …
[HTML][HTML] Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity
A compact protein with a size of< 1,000 amino acids, the CRISPR-associated protein CasX
is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a …
is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a …
Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria
D Collias, E Vialetto, J Yu, K Co, ÉH Almási… - Nature …, 2023 - nature.com
Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to
counter-select against unedited cells. However, editing normally requires efficient …
counter-select against unedited cells. However, editing normally requires efficient …
Hairpin‐spacer crRNA‐enhanced CRISPR/Cas13a system promotes the specificity of single nucleotide polymorphism (SNP) identification
Y Ke, S Huang, B Ghalandari, S Li… - Advanced …, 2021 - Wiley Online Library
The Cas13a system has great potential in RNA interference and molecular diagnostic fields.
However, lacking guidelines for crRNA design hinders practical applications of the Cas13a …
However, lacking guidelines for crRNA design hinders practical applications of the Cas13a …
Limitations of dual-single guide RNA CRISPR strategies for the treatment of central nervous system genetic disorders
F Duarte, G Vachey, NS Caron, M Sipion… - Human Gene …, 2023 - liebertpub.com
Huntington's disease (HD) is a fatal neurodegenerative disorder caused by a toxic gain-of-
function CAG expansion in the first exon of the huntingtin (HTT) gene. The monogenic …
function CAG expansion in the first exon of the huntingtin (HTT) gene. The monogenic …