Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some
features of solid tumors, such as their spatial architecture, physiological responses …

The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

For the 1.7 million patients per year in the US who receive a new cancer diagnosis,
treatment decisions are largely based on histopathological specimen examinations …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Introduction In vivo imaging provides a window into the spatially complex, rapidly evolving
physiology of the cell that structural imaging alone cannot. However, observing this …

Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …