Following its transcription, RNA can be modified by> 170 chemically distinct types of
modifications—the epitranscriptome. In recent years, there have been substantial efforts to …

Advances in the depth and quality of transcriptome sequencing have revealed many new
classes of long noncoding RNAs (lncRNAs). lncRNA classification has mushroomed to …

De novo assembly of RNA-seq data enables researchers to study transcriptomes without the
need for a genome sequence; this approach can be usefully applied, for instance, in …

RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods
to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing …

Modifications of RNA affect its function and stability. RNA editing is unique among these
modifications because it not only alters the cellular fate of RNA molecules but also alters …

Next-generation sequencing allows the analysis of genomes, including those representing
disease states. However, the causes of most disorders are multifactorial, and systems-level …

In recent years, major breakthroughs in RNA-modification-mediated regulation of gene
expression have been made, leading to the emerging field of epitranscriptomics. Our …

RNA molecules transmit the information encoded in the genome and generally reflect its
content. Adenosine-to-inosine (A-to-I) RNA editing by ADAR proteins converts a genomically …

RNA editing by adenosine deaminases changes the information encoded in the mRNA from
its genomic blueprint. Editing of protein-coding sequences can introduce novel, functionally …

The chemical identity of RNA molecules beyond the four standard ribonucleosides has
fascinated scientists since pseudouridine was characterized as the “fifth” ribonucleotide in …