Electroporation-based applications in biotechnology

T Kotnik, W Frey, M Sack, SH Meglič, M Peterka… - Trends in …, 2015 - cell.com
Electroporation is already an established technique in several areas of medicine, but many
of its biotechnological applications have only started to emerge; we review here some of the …

Cell membrane electroporation-Part 1: The phenomenon

T Kotnik, P Kramar, G Pucihar… - IEEE Electrical …, 2012 - ieeexplore.ieee.org
Each biological cell, trillions of which build our bodies, is enveloped by its plasma
membrane. Composed largely of a bilayer (double layer) of lipids just two molecules thick …

Chapter seven electroporation of planar lipid bilayers and membranes

M Pavlin, T Kotnik, D Miklavčič, P Kramar… - Advances in planar lipid …, 2008 - Elsevier
Strong external electric field can destabilize membranes and induce formation of pores thus
increasing membrane permeability. The phenomenon is known as membrane …

Electroporating fields target oxidatively damaged areas in the cell membrane

PT Vernier, ZA Levine, YH Wu, V Joubert, MJ Ziegler… - PloS one, 2009 - journals.plos.org
Reversible electropermeabilization (electroporation) is widely used to facilitate the
introduction of genetic material and pharmaceutical agents into living cells. Although …

Numerical simulation of molecular uptake via electroporation

J Li, H Lin - Bioelectrochemistry, 2011 - Elsevier
A numerical study of electroporation-mediated molecular delivery is presented. The model
consists of the Nernst–Planck equations for species transport, coupled with an asymptotic …

Enhancement of cell membrane permeability by using charged nanoparticles and a weak external electric field

H Nakamura, T Okamura, M Tajima… - Physical Chemistry …, 2023 - pubs.rsc.org
Because the cell membrane is the main barrier of intracellular delivery, it is important to
facilitate and control the translocation of extracellular compounds across it. Our earlier …

Electroporation of archaeal lipid membranes using MD simulations

A Polak, M Tarek, M Tomšič, J Valant, NP Ulrih… - …, 2014 - Elsevier
Molecular dynamics (MD) simulations were used to investigate the electroporation of
archaeal lipid bilayers when subjected to high transmembrane voltages induced by a …

Tailored polymeric membranes for Mycobacterium smegmatis porin A (MspA) based biosensors

D Morton, S Mortezaei, S Yemenicioglu… - Journal of Materials …, 2015 - pubs.rsc.org
Nanopores based on protein channels inserted into lipid membranes have paved the way
towards a wide-range of inexpensive biosensors, especially for DNA sequencing. A key …

Effect of nanosecond pulsed electric fields (nsPEFs) on coronavirus survival

JC Cantu, RA Barnes, BM Gamboa, AS Keister… - AMB Express, 2023 - Springer
Previous work demonstrated inactivation of influenza virus by GHz frequency
electromagnetic fields. Despite theoretical and experimental results, the underlying …

Effect of the cholesterol on electroporation of planar lipid bilayer

P Kramar, D Miklavčič - Bioelectrochemistry, 2022 - Elsevier
Electroporation threshold depends on the membrane composition, with cholesterol being
one of its key components already studied in the past, but the results were inconclusive. The …