The next generation of CRISPR–Cas technologies and applications
A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
CRISPR-Cas for genome editing: classification, mechanism, designing and applications
S Bhatia, SK Yadav - International Journal of Biological Macromolecules, 2023 - Elsevier
Clustered regularly interspersed short pallindromic repeats (CRISPR) and CRISPR
associated proteins (Cas) system (CRISPR-Cas) came into light as prokaryotic defence …
associated proteins (Cas) system (CRISPR-Cas) came into light as prokaryotic defence …
How to measure and evaluate binding affinities
I Jarmoskaite, I AlSadhan, PP Vaidyanathan… - Elife, 2020 - elifesciences.org
Quantitative measurements of biomolecule associations are central to biological
understanding and are needed to build and test predictive and mechanistic models. Given …
understanding and are needed to build and test predictive and mechanistic models. Given …
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice
Antibiotic treatments have detrimental effects on the microbiome and lead to antibiotic
resistance. To develop a phage therapy against a diverse range of clinically relevant …
resistance. To develop a phage therapy against a diverse range of clinically relevant …
[HTML][HTML] Biology and applications of CRISPR systems: harnessing nature's toolbox for genome engineering
Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
CRISPR-Cas immunity in prokaryotes
LA Marraffini - Nature, 2015 - nature.com
Prokaryotic organisms are threatened by a large array of viruses and have developed
numerous defence strategies. Among these, only clustered, regularly interspaced short …
numerous defence strategies. Among these, only clustered, regularly interspaced short …
CRISPR-Cas: biology, mechanisms and relevance
F Hille, E Charpentier - … of the royal society B: biological …, 2016 - royalsocietypublishing.org
Prokaryotes have evolved several defence mechanisms to protect themselves from viral
predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their …
predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their …
Classification and nomenclature of CRISPR-Cas systems: where from here?
KS Makarova, YI Wolf, EV Koonin - The CRISPR journal, 2018 - liebertpub.com
As befits an immune mechanism, CRISPR-Cas systems are highly variable with respect to
Cas protein sequences, gene composition, and organization of the genomic loci. Optimal …
Cas protein sequences, gene composition, and organization of the genomic loci. Optimal …
Molecular mechanisms of CRISPR-Cas immunity in bacteria
PM Nussenzweig, LA Marraffini - Annual review of genetics, 2020 - annualreviews.org
Prokaryotes have developed numerous defense strategies to combat the constant threat
posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced …
posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced …
A compact Cascade–Cas3 system for targeted genome engineering
CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …