Free-energy profiles for membrane permeation of compounds calculated using rare-event sampling methods

R Harada, R Morita, Y Shigeta - Journal of Chemical Information …, 2022 - ACS Publications
The free-energy profile of a compound is an essential measurement in evaluating the
membrane permeation process by means of theoretical methods. Computationally …

The role of ATP in solubilizing RNA‐binding protein fused in sarcoma

H Aida, Y Shigeta, R Harada - Proteins: Structure, Function, and …, 2022 - Wiley Online Library
Intrinsically disordered protein (IDP) plays an important role in liquid–liquid phase
separation (LLPS). RNA‐binding protein fused in sarcoma (FUS) is a well‐studied IDP that …

Simple, yet powerful methodologies for conformational sampling of proteins

R Harada, Y Takano, T Baba, Y Shigeta - Physical chemistry chemical …, 2015 - pubs.rsc.org
Several biological functions, such as molecular recognition, enzyme catalysis, signal
transduction, allosteric regulation, and protein folding, are strongly related to conformational …

Design of electron-donating group substituted 2-PAM analogs as antidotes for organophosphate insecticide poisoning

N Kongkaew, K Hengphasatporn, Y Injongkol… - RSC …, 2023 - pubs.rsc.org
The use of organophosphate (OPs) pesticides is widespread in agriculture and horticulture,
but these chemicals can be lethal to humans, causing fatalities and deaths each year. The …

Determination of the Association between Mesotrione Sensitivity and Conformational Change of 4-Hydroxyphenylpyruvate Dioxygenase via Free-Energy Analyses

Y Munei, K Hengphasatporn, Y Hori… - Journal of Agricultural …, 2023 - ACS Publications
One widely known herbicide target is 4-hydroxyphenylpyruvate dioxygenase (HPPD). Avena
sativa HPPD is less sensitive to mesotrione (herbicide) than Arabidopsis thaliana HPPD …

Protein folding pathways extracted by OFLOOD: Outlier FLOODing method

R Harada, T Nakamura, Y Takano… - Journal of …, 2015 - Wiley Online Library
The Outlier FLOODing method (OFLOOD) is proposed as an efficient conformational
sampling method to extract biologically rare events such as protein folding. In OFLOOD …

A Structural Refinement Technique for Protein-RNA Complexes Based on a Combination of AI-based Modeling and Flexible Docking: A Study of Musashi-1 Protein

N Darai, K Hengphasatporn… - Bulletin of the …, 2023 - academic.oup.com
An efficient structural refinement technique for protein-RNA complexes is proposed based
on a combination of AI-based modeling and flexible docking. Specifically, an enhanced …

Efficient conformational search based on structural dissimilarity sampling: applications for reproducing structural transitions of proteins

R Harada, Y Shigeta - Journal of Chemical Theory and …, 2017 - ACS Publications
Structural Dissimilarity Sampling (SDS) is proposed as an efficient conformational search
method to promote structural transitions essential for the biological functions of proteins. In …

Structural Validation by the G-Factor Properly Regulates Boost Potentials Imposed in Conformational Sampling of Proteins

T Yasuda, R Morita, Y Shigeta… - Journal of Chemical …, 2022 - ACS Publications
Free energy landscapes (FELs) of proteins are indispensable for evaluating thermodynamic
properties. Molecular dynamics (MD) simulation is a computational method for calculating …

Ligand binding path sampling based on parallel cascade selection molecular dynamics: LB-PaCS-MD

H Aida, Y Shigeta, R Harada - Materials, 2022 - mdpi.com
Parallel cascade selection molecular dynamics (PaCS-MD) is a rare-event sampling method
that generates transition pathways between a reactant and product. To sample the transition …