The progress of genetic engineering in the 1970s brought about a paradigm shift in genome
editing technology. The clustered regularly interspaced short palindromic repeats/CRISPR …

Genetic interactions mediate the emergence of phenotype from genotype, but technologies
for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we …

Exploiting cancer vulnerabilities is critical for the discovery of anticancer drugs. However,
tumor suppressors cannot be directly targeted because of their loss of function. To uncover …

Redox metabolism plays a central role in the cellular metabolism network, involves catabolic
and anabolic reactions of diverse biomass, and determines the redox state of cells. It can be …

Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological
cellular functions and drive devastating human diseases. Profiling PPI networks can …

Alternative splicing is crucial for cancer progression and can be targeted pharmacologically,
yet identifying driver exons genome-wide remains challenging. We propose identifying such …

Chromatin regulators are frequently mutated in human cancer and are attractive drug
targets. They include diverse proteins that share functional domains and assemble into …

Abstract PICKLES (https://pickles. hart-lab. org) is an updated web interface to a freely
available database of genome-scale CRISPR knockout fitness screens in human cell lines …

Background Coessentiality networks derived from CRISPR screens in cell lines provide a
powerful framework for identifying functional modules in the cell and for inferring the roles of …

Simple Summary High-throughput CRISPR screening provides an unbiased way for
functional genomic studies in hematological malignancies. This approach has been used to …