Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique

B Buszewski, S Noga - Analytical and bioanalytical chemistry, 2012 - Springer
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to
effectively separate small polar compounds on polar stationary phases. The purpose of this …

Systems level studies of mammalian metabolomes: the roles of mass spectrometry and nuclear magnetic resonance spectroscopy

WB Dunn, DI Broadhurst, HJ Atherton… - Chemical society …, 2011 - pubs.rsc.org
The study of biological systems in a holistic manner (systems biology) is increasingly being
viewed as a necessity to provide qualitative and quantitative descriptions of the emergent …

MZmine 2: modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data

T Pluskal, S Castillo, A Villar-Briones, M Orešič - BMC bioinformatics, 2010 - Springer
Background Mass spectrometry (MS) coupled with online separation methods is commonly
applied for differential and quantitative profiling of biological samples in metabolomic as well …

Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry

WB Dunn, D Broadhurst, P Begley, E Zelena… - Nature protocols, 2011 - nature.com
Metabolism has an essential role in biological systems. Identification and quantitation of the
compounds in the metabolome is defined as metabolic profiling, and it is applied to define …

[HTML][HTML] The human urine metabolome

S Bouatra, F Aziat, R Mandal, AC Guo, MR Wilson… - PloS one, 2013 - journals.plos.org
Urine has long been a “favored” biofluid among metabolomics researchers. It is sterile, easy-
to-obtain in large volumes, largely free from interfering proteins or lipids and chemically …

XCMS Online: a web-based platform to process untargeted metabolomic data

R Tautenhahn, GJ Patti, D Rinehart… - Analytical …, 2012 - ACS Publications
Recently, interest in untargeted metabolomics has become prevalent in the general scientific
community among an increasing number of investigators. The majority of these …

Global metabolic profiling procedures for urine using UPLC–MS

EJ Want, ID Wilson, H Gika, G Theodoridis, RS Plumb… - Nature protocols, 2010 - nature.com
The production of'global'metabolite profiles involves measuring low molecular-weight
metabolites (< 1 kDa) in complex biofluids/tissues to study perturbations in response to …

Double-check: validation of diagnostic statistics for PLS-DA models in metabolomics studies

E Szymańska, E Saccenti, AK Smilde, JA Westerhuis - Metabolomics, 2012 - Springer
Abstract Partial Least Squares-Discriminant Analysis (PLS-DA) is a PLS regression method
with a special binary 'dummy'y-variable and it is commonly used for classification purposes …

Stationary and mobile phases in hydrophilic interaction chromatography: a review

P Jandera - Analytica chimica acta, 2011 - Elsevier
Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase
liquid chromatography separations of polar, weakly acidic or basic samples. In principle, this …

Column characterization and selection systems in reversed-phase high-performance liquid chromatography

P Žuvela, M Skoczylas, J Jay Liu, T Ba̧czek… - Chemical …, 2019 - ACS Publications
Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular
chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes …