The inkjet technique has the capability of generating droplets in the picoliter volume range,
firing thousands of times in a few seconds and printing in the noncontact manner. Since its …

For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …

The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …

Learning to read and write the transcriptional regulatory code is of central importance to
progress in genetic analysis and engineering. Here we describe a massively parallel …

The ease of genetic manipulation, low cost, rapid growth and number of previous studies
have made Escherichia coli one of the most widely used microorganism species for …

Heterologous expression is the main approach for recombinant protein production ingenetic
synthesis, for which codon optimization is necessary. The existing optimization methods are …

Genome‐modification technologies enable the rational engineering and perturbation of
biological systems. Historically, these methods have been limited to gene insertions or …

Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …

We provide an overview of current approaches to DNA-based storage system design and of
accompanying synthesis, sequencing and editing methods. We also introduce and analyze …

Synthetic biology aims to create functional devices, systems and organisms with novel and
useful functions on the basis of catalogued and standardized biological building blocks …