An explosion in single-cell technologies has revealed a previously underappreciated
heterogeneity of cell types and novel cell-state associations with sex, disease, development …

New technologies have enabled the investigation of biology and human health at an
unprecedented scale and in multiple dimensions. These dimensions include a myriad of …

Understanding global communications among cells requires accurate representation of cell-
cell signaling links and effective systems-level analyses of those links. We construct a …

One of the most fundamental questions in biology is what types of cells form different tissues
and organs in a functionally coordinated fashion. Larger-scale single-cell sequencing and …

The lineage relationships among the hundreds of cell types generated during development
are difficult to reconstruct. A recent method, GESTALT, used CRISPR–Cas9 barcode editing …

Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …

Reconstructing lineage relationships between cells within a tissue or organism is a long-
standing aim in biology. Traditionally, lineage tracing has been achieved through the …

Highlights•Technologies such as single-cell RNA sequencing have rapidly progressed in
recent years.•Various methods exist for preparing libraries for sequencing, each with its own …

Spatial transcriptomics enables the simultaneous measurement of morphological features
and transcriptional profiles of the same cells or regions in tissues. Here we present multi …

How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its
identity remains poorly understood. We developed an approach to distinguish between …