A guide to studying protein aggregation

JAJ Housmans, G Wu, J Schymkowitz… - The FEBS …, 2023 - Wiley Online Library
Disrupted protein folding or decreased protein stability can lead to the accumulation of
(partially) un‐or misfolded proteins, which ultimately cause the formation of protein …

Theory and applications of differential scanning fluorimetry in early-stage drug discovery

K Gao, R Oerlemans, MR Groves - Biophysical reviews, 2020 - Springer
Differential scanning fluorimetry (DSF) is an accessible, rapid, and economical biophysical
technique that has seen many applications over the years, ranging from protein folding state …

Rapid alkalinization factor 22 has a structural and signalling role in root hair cell wall assembly

S Schoenaers, HK Lee, M Gonneau, E Faucher… - Nature Plants, 2024 - nature.com
Pressurized cells with strong walls make up the hydrostatic skeleton of plants. Assembly and
expansion of such stressed walls depend on a family of secreted RAPID ALKALINIZATION …

Plant cell wall patterning and expansion mediated by protein-peptide-polysaccharide interaction

S Moussu, HK Lee, KT Haas, C Broyart, U Rathgeb… - Science, 2023 - science.org
Assembly of cell wall polysaccharides into specific patterns is required for plant growth. A
complex of RAPID ALKALINIZATION FACTOR 4 (RALF4) and its cell wall–anchored …

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD

U Gupta, S Ghosh, CT Wallace, P Shang, Y **n… - Autophagy, 2023 - Taylor & Francis
In dry age-related macular degeneration (AMD), LCN2 (lipocalin 2) is upregulated. Whereas
LCN2 has been implicated in AMD pathogenesis, the mechanism remains unknown. Here …

Chemically stable fluorescent proteins for advanced microscopy

BC Campbell, MG Paez-Segala, LL Looger… - Nature …, 2022 - nature.com
We report the rational engineering of a remarkably stable yellow fluorescent protein
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …

Small-molecule inhibitors of the coronavirus spike: ACE2 protein–protein interaction as blockers of viral attachment and entry for SARS-CoV-2

D Bojadzic, O Alcazar, J Chen, ST Chuang… - ACS infectious …, 2021 - ACS Publications
Inhibitors of the protein–protein interaction (PPI) between the SARS-CoV-2 spike protein
and human ACE2 (hACE2), which acts as a ligand–receptor pair that initiates the viral …

Epigallocatechin-3-gallate, an active ingredient of Traditional Chinese Medicines, inhibits the 3CLpro activity of SARS-CoV-2

A Du, R Zheng, C Disoma, S Li, Z Chen, S Li… - International journal of …, 2021 - Elsevier
SARS-CoV-2 is the etiological agent responsible for the ongoing pandemic of coronavirus
disease 2019 (COVID-19). The main protease of SARS-CoV-2, 3CLpro, is an attractive …

Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2

Y Kim, J Wower, N Maltseva, C Chang… - Communications …, 2021 - nature.com
Abstract SARS-CoV-2 Nsp15 is a uridine-specific endoribonuclease with C-terminal catalytic
domain belonging to the EndoU family that is highly conserved in coronaviruses. As …

Counteraction of stability-activity trade-off of Nattokinase through flexible region shifting

J Luo, C Song, W Cui, L Han, Z Zhou - Food Chemistry, 2023 - Elsevier
The widespread trade-off between stability and activity severely limits enzyme evolution.
Although some progresses have been made to overcome this limitation, the counteraction …