Chemical synthesis of human proteoforms and application in biomedicine

H Ai, M Pan, L Liu - ACS Central Science, 2024 - ACS Publications
Limited understanding of human proteoforms with complex posttranslational modifications
and the underlying mechanisms poses a major obstacle to research on human health and …

Recent advances in chemical protein synthesis: method developments and biological applications

S Dong, JS Zheng, Y Li, H Wang, G Chen… - Science China …, 2024 - Springer
The central dogma of modern biology underscores the pivotal roles proteins play in diverse
biological processes, the study of which necessitates advanced methods to produce …

L‐Glycosidase‐Cleavable Natural Glycans Facilitate the Chemical Synthesis of Correctly Folded Disulfide‐Bonded D‐Proteins

W Shi, T Wang, Z Yang, Y Ren, D Han… - Angewandte …, 2024 - Wiley Online Library
D‐peptide ligands can be screened for therapeutic potency and enzymatic stability using
synthetic mirror‐image proteins (D‐proteins), but efficient acquisition of these D‐proteins …

Mirror-image protein and peptide drug discovery through mirror-image phage display

YK Qi, JS Zheng, L Liu - Chem, 2024 - cell.com
Mirror-image proteins and peptides composed of D-amino acids are garnering increasing
attention as diagnostic agents and drug candidates due to their higher stability and lower …

Ligation embedding aggregation disruptor strategy enables the chemical synthesis of PD-1 immunoglobulin and extracellular domains

H Wu, T Wei, WL Ngai, H Zhou, X Li - Journal of the American …, 2022 - ACS Publications
Chemical synthesis of proteins with aggregable or colloidal peptide segments presents a
formidable task, as such peptides prove to be difficult for both solid-phase peptide synthesis …

Total synthesis of interleukin-2 via a tunable backbone modification strategy

H Wu, Y Tan, WL Ngai, X Li - Chemical Science, 2023 - pubs.rsc.org
Chemical synthesis of hydrophobic proteins presents a formidable task as they are often
difficultly achieved via peptide synthesis, purification, and peptide ligation. Thus, peptide …

Sulfur-containing peptides: Synthesis and application in the discovery of potential drug candidates

Y Liao, M Wang, X Jiang - Current Opinion in Chemical Biology, 2023 - Elsevier
Peptides act as biological mediators and play a key role of various physiological activities.
Sulfur-containing peptides are widely used in natural products and drug molecules due to …

Recent advances in chemical synthesis of O-linked glycopeptides and glycoproteins: An advanced synthetic tool for exploring the biological realm

J Zhao, F Ye, P Huang, P Wang - Current Opinion in Chemical Biology, 2023 - Elsevier
Glycoproteins play crucial roles in various biological processes. To investigate the
relationship between glycan structure and function, researchers have employed various …

Photocaging of Activity‐Based Ubiquitin Probes via a C‐Terminal Backbone Modification Strategy

Y Wang, J Chen, X Hua, X Meng, H Cai… - Angewandte …, 2022 - Wiley Online Library
Photocaged, activity‐based ubiquitin (Ub) probes (Ub‐ABPs) have been developed for the
time‐resolved probing of deubiquitinating enzyme (DUB) activities, but many Ub‐ABPs are …

Revealing the extracellular function of HMGB1 N-terminal region acetylation assisted by a protein semi-synthesis approach

T Wei, J Liu, C Li, Y Tan, R Wei, J Wang, H Wu, Q Li… - Chemical …, 2023 - pubs.rsc.org
HMGB1 (high-mobility group box 1) is a non-histone chromatin-associated protein that has
been widely reported as a representative damage-associated molecular pattern (DAMP) …