In the era of functional genomics, the need for tools to perform large-scale targeted and
random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal …

Filamentous fungi have long been used for production of a range of valuable products; with
the advent of molecular biology, it became apparent that these fungi possess considerable …

The ability to artificially control transcription is essential both to the study of gene function
and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA …

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …

Streptomycetes are high G+ C Gram-positive, antibiotic-producing, mycelial soil bacteria.
The 8.7-Mb Streptomyces coelicolor genome was previously sequenced by using an …

High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …

Gene replacement via homologous double crossover in filamentous fungi requires relatively
long (preferentially> 0.5 kb) flanking regions of the target gene. For this reason, gene …

The low rate of homologous recombination exhibited by wild-type strains of filamentous
fungi has hindered development of high-throughput gene knockout procedures for this …

Differentiation and secondary metabolism are correlated processes in fungi that respond to
light. In Aspergillus nidulans, light inhibits sexual reproduction as well as secondary …

We describe a rapid method for the production of fusion PCR products that can be used,
generally without band purification, to transform Aspergillus nidulans. This technique can be …