Preparation of tissues for DNA flow cytometric analysis
JT Thornthwaite, EV Sugarbaker… - Cytometry: The Journal …, 1980 - Wiley Online Library
A method for measuring DNA in tissue cells by flow cytometry utilizing a one step
combination nuclear isolation‐DNA fluorochrome staining procedure is described. A variety …
combination nuclear isolation‐DNA fluorochrome staining procedure is described. A variety …
A review of in vitro and in vivo culture techniques for the study of pancreatic carcinogenesis
RT Jones, EA Hudson, JH Resau - Cancer, 1981 - Wiley Online Library
Techniques have been developed for long‐term organ explant culture of bovine, hamster,
and human pancreatic ducts in enriched medium. The explants were then exposed to …
and human pancreatic ducts in enriched medium. The explants were then exposed to …
Optimization in osmolality and range of density of a continuous ficoll-sodium-diatrizoate gradient for isopycnic purification of isolated human islets
M Eckhard, D Brandhorst, H Brandhorst… - Transplantation …, 2004 - Elsevier
INTRODUCTION: According to previous estimates from large animals and man, a minimum
of approximately 5000 to 6000 engrafted islet equivalents (IEQ)/kg recipient weight is critical …
of approximately 5000 to 6000 engrafted islet equivalents (IEQ)/kg recipient weight is critical …
[22] Cell separation by gradient centrifugation methods
TG Pretlow, TP Pretlow - Methods in enzymology, 1989 - Elsevier
Publisher Summary Methods for the purification of individual kinds of cells have increased
markedly in variety and sophistication during the last two decades. In addition to gradient …
markedly in variety and sophistication during the last two decades. In addition to gradient …
Characterization of bovine pancreatic ductal cells isolated by a perfusion-digestion technique
T Sato, M Sato, EA Hudson, RT Jones - In vitro, 1983 - Springer
Bovine pancreatic ductal cells isolated by perfusing an enzyme solution into the lumen of the
main duct were obtained as sheets of cells. Morphologic features of these cells were those …
main duct were obtained as sheets of cells. Morphologic features of these cells were those …
Separation of epithelial cells from suspensions of cells from the hamster parotid gland in an isokinetic density gradient of Ficoll in tissue culture medium
DW Aspray, A Pitts, TG Pretlow II - Analytical Biochemistry, 1975 - Elsevier
Epithelial cells were separated from suspensions of hamster parotid cells by velocity
sedimentation in an isokinetic gradient and by isopycnic sedimentation. Epithelial cells were …
sedimentation in an isokinetic gradient and by isopycnic sedimentation. Epithelial cells were …
Separation of viable cells by velocity sedimentation in an isokinetic gradient of ficoll in tissue culture medium
TG Pretlow, TP Pretlow - Methods of Cell Separation, 1977 - Springer
Recent progress in the development of methods for the separation of individual kinds of cells
has facilitated the characterization of individual kinds of cells from complex mixtures of cells …
has facilitated the characterization of individual kinds of cells from complex mixtures of cells …
Separation and characterization of neoplastic cell subpopulations of a transplantable rat pancreatic acinar carcinoma
MJ Becich, JK Reddy - Cancer Research, 1982 - aacrjournals.org
The transplantable pancreatic acinar carcinoma established in F344 rats demonstrates
heterogeneity of cytodifferentiation with cell types ranging from those containing no …
heterogeneity of cytodifferentiation with cell types ranging from those containing no …
Separation of cells having histochemically demonstrable glucose-6-phosphatase from suspensions of hamster kidney cells in an isokinetic density gradient of Ficoll in …
TG Pretlow, J Jones, S Dow - The American Journal of …, 1974 - pmc.ncbi.nlm.nih.gov
Suspensions of enzymatically disaggregated hamster kidney cells were separated primarily
according to differences in diameter using velocity sedimentation in a previously described …
according to differences in diameter using velocity sedimentation in a previously described …
Separation of acinar cells from lactating mammary gland using velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium
TG Pretlow, SR Dow, TM Murad… - The American Journal …, 1974 - pmc.ncbi.nlm.nih.gov
This report describes a procedure for the purification of acinar cells from the disaggregated
lactating mammary gland of the rat. Following disaggregation of the 15 days postpartum …
lactating mammary gland of the rat. Following disaggregation of the 15 days postpartum …