Structure and mechanism of the Red recombination system of bacteriophage λ
BJ Caldwell, CE Bell - Progress in biophysics and molecular biology, 2019 - Elsevier
While much of this volume focuses on mammalian DNA repair systems that are directly
involved in genome stability and cancer, it is important to still be mindful of model systems …
involved in genome stability and cancer, it is important to still be mindful of model systems …
Bacteriophage λ exonuclease and a 5′-phosphorylated DNA guide allow PAM-independent targeting of double-stranded nucleic acids
S Fu, J Li, J Chen, L Zhang, J Liu, H Liu, X Su - Nature Biotechnology, 2024 - nature.com
Sequence-specific recognition of double-stranded nucleic acids is essential for molecular
diagnostics and in situ imaging. Clustered regularly interspaced short palindromic repeats …
diagnostics and in situ imaging. Clustered regularly interspaced short palindromic repeats …
Half a century of bacteriophage lambda recombinase: In vitro studies of lambda exonuclease and Red‐beta annealase
DNA recombination, replication, and repair are intrinsically interconnected processes. From
viruses to humans, they are ubiquitous and essential to all life on Earth. Single‐strand …
viruses to humans, they are ubiquitous and essential to all life on Earth. Single‐strand …
Computationally identifying hot spots in protein-DNA binding interfaces using an ensemble approach
Background Protein-DNA interaction governs a large number of cellular processes, and it
can be altered by a small fraction of interface residues, ie, the so-called hot spots, which …
can be altered by a small fraction of interface residues, ie, the so-called hot spots, which …
Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli …
BJ Caldwell, E Zakharova, GT Filsinger… - Nucleic acids …, 2019 - academic.oup.com
Bacteriophage λ encodes a DNA recombination system that includes a 5′-3′ exonuclease
(λ Exo) and a single strand annealing protein (Redβ). The two proteins form a complex that …
(λ Exo) and a single strand annealing protein (Redβ). The two proteins form a complex that …
Noncanonical substrate preference of lambda exonuclease for 5′-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic …
T Wu, Y Yang, W Chen, J Wang, Z Yang… - Nucleic Acids …, 2018 - academic.oup.com
Lambda exonuclease (λ exo) plays an important role in the resection of DNA ends for DNA
repair. Currently, it is also a widely used enzymatic tool in genetic engineering, DNA-binding …
repair. Currently, it is also a widely used enzymatic tool in genetic engineering, DNA-binding …
Digestion of dynamic substrate by exonuclease reveals high single-mismatch selectivity
Y Yu, L Ma, L Li, Y Deng, L Xu, H Liu, L **ao… - Analytical …, 2018 - ACS Publications
The distinctive nuclease activity toward nucleic acid substrates enables various applications
in analytical chemistry and dynamic DNA nanotechnology. λ Exonuclease is a widely used …
in analytical chemistry and dynamic DNA nanotechnology. λ Exonuclease is a widely used …
[HTML][HTML] Half a century after their discovery: Structural insights into exonuclease and annealase proteins catalyzing recombineering
Recombineering is an essential tool for molecular biologists, allowing for the facile and
efficient manipulation of bacterial genomes directly in cells without the need for costly and …
efficient manipulation of bacterial genomes directly in cells without the need for costly and …
Allosteric ring assembly and chemo-mechanical melting by the interaction between 5′-phosphate and λ exonuclease
J Yoo, G Lee - Nucleic acids research, 2015 - academic.oup.com
Phosphates along the DNA function as chemical energy frequently used by nucleases to
drive their enzymatic reactions. Exonuclease functions as a machine that converts chemical …
drive their enzymatic reactions. Exonuclease functions as a machine that converts chemical …
[BOOK][B] Structural, biophysical, and biochemical characterization of the single-strand annealing system from bacteriophage λ
BJ Caldwell - 2021 - search.proquest.com
Bacteriophage λ, one of the earliest model systems used for studying DNA recombination,
encodes a simple" SynExo"(synaptase-exonuclease) recombination system for the repair of …
encodes a simple" SynExo"(synaptase-exonuclease) recombination system for the repair of …