Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking
The exploration of protein structure and function stands at the forefront of life science and
represents an ever‐expanding focus in the development of proteomics. As mass …
represents an ever‐expanding focus in the development of proteomics. As mass …
Exploring protein conformations with limited proteolysis coupled to mass spectrometry
C Van Leene, L Van Moortel, K De Bosscher… - Trends in Biochemical …, 2024 - cell.com
Limited proteolysis coupled to mass spectrometry (LiP-MS) has emerged as a powerful
proteomic tool for studying protein conformations. Since its introduction in 2014, LiP-MS has …
proteomic tool for studying protein conformations. Since its introduction in 2014, LiP-MS has …
The ASC inflammasome adapter governs SAA-derived protein aggregation in inflammatory amyloidosis
M Losa, M Emmenegger, P De Rossi… - EMBO Molecular …, 2024 - embopress.org
Extracellularly released molecular inflammasome assemblies-ASC specks-cross-seed Aβ
amyloid in Alzheimer's disease. Here we show that ASC governs the extent of inflammation …
amyloid in Alzheimer's disease. Here we show that ASC governs the extent of inflammation …
Structural basis of calmodulin modulation of the rod cyclic nucleotide-gated channel
DCA Barret, D Schuster… - Proceedings of the …, 2023 - National Acad Sciences
Calmodulin (CaM) regulates many ion channels to control calcium entry into cells, and
mutations that alter this interaction are linked to fatal diseases. The structural basis of CaM …
mutations that alter this interaction are linked to fatal diseases. The structural basis of CaM …
Global profiling of protein complex dynamics with an experimental library of protein interaction markers
Methods to systematically monitor protein complex dynamics are needed. We introduce
serial ultrafiltration combined with limited proteolysis-coupled mass spectrometry (FLiP–MS) …
serial ultrafiltration combined with limited proteolysis-coupled mass spectrometry (FLiP–MS) …
From cancer therapy to cardiac safety: the role of proteostasis in drug-induced cardiotoxicity
X Qian, M Yao, J Xu, N Dong, S Chen - Frontiers in Pharmacology, 2024 - frontiersin.org
Drug-induced cardiotoxicity (DICT) poses a significant challenge in the prognosis of cancer
patients, particularly with the use of antineoplastic agents like anthracyclines and targeted …
patients, particularly with the use of antineoplastic agents like anthracyclines and targeted …
Mass spectrometry-based methods for characterizing transient protein–protein interactions
The dynamic associations of transient protein–protein interactions (PPIs) are critical
mediators of myriad biochemical processes. These specific, low-affinity interactions are often …
mediators of myriad biochemical processes. These specific, low-affinity interactions are often …
[HTML][HTML] Beyond Misfolding: A New Paradigm for the Relationship Between Protein Folding and Aggregation
SI Choi, Y **, Y Choi, BL Seong - International Journal of Molecular …, 2024 - mdpi.com
Aggregation is intricately linked to protein folding, necessitating a precise understanding of
their relationship. Traditionally, aggregation has been viewed primarily as a sequential …
their relationship. Traditionally, aggregation has been viewed primarily as a sequential …
Regulatory sites of CaM-sensitive adenylyl cyclase AC8 revealed by cryo-EM and structural proteomics
Membrane adenylyl cyclase AC8 is regulated by G proteins and calmodulin (CaM),
mediating the crosstalk between the cAMP pathway and Ca2+ signalling. Despite the …
mediating the crosstalk between the cAMP pathway and Ca2+ signalling. Despite the …
Regulatory sites of CaM-sensitive adenylyl cyclase AC8 revealed by cryo-EM and structural proteomics
Membrane adenylyl cyclase AC8 is regulated by G proteins and calmodulin (CaM),
mediating the crosstalk between the cAMP pathway and Ca2+ signalling. Despite the …
mediating the crosstalk between the cAMP pathway and Ca2+ signalling. Despite the …