Current metabolic engineering strategies for photosynthetic bioproduction in cyanobacteria

A Satta, L Esquirol, BE Ebert - Microorganisms, 2023 - mdpi.com
Cyanobacteria are photosynthetic microorganisms capable of using solar energy to convert
CO2 and H2O into O2 and energy-rich organic compounds, thus enabling sustainable …

RNA compensation: a positive feedback insulation strategy for RNA-based transcription networks

B Liu, C Cuba Samaniego, M Bennett… - ACS Synthetic …, 2022 - ACS Publications
The lack of signaling modularity of biomolecular systems poses major challenges toward
engineering complex networks. Directional signaling between an upstream and a …

Cell-free transcription-coupled CRISPR/Cas12a assay for prototy** cyanobacterial promoters

YN Choi, YR Shin, JM Park, JW Lee - ACS Synthetic Biology, 2021 - ACS Publications
Cyanobacteria are promising microbial hosts for the production of diverse biofuels and
biochemicals. However, compared to other model microbial hosts such as Escherichia coli …

Engineering quorum sensing-based genetic circuits enhances growth and productivity robustness of industrial E. coli at low pH

X Yan, A Bu, Y Yuan, X Zhang, Z Lin, X Yang - Microbial Cell Factories, 2024 - Springer
Background Microbial organisms hold significant potential for converting renewable
substrates into valuable chemicals. Low pH fermentation in industrial settings offers key …

Entangled adaptive landscapes facilitate the evolution of gene regulation by exaptation

CA Westmann, L Goldbach, A Wagner - bioRxiv, 2024 - biorxiv.org
Exaptation, the co–option of existing traits for new functions, is a central process in
Darwinian evolution. However, the molecular changes leading to exaptations remain …

Prevention of ribozyme catalysis through cDNA synthesis enables accurate RT-qPCR measurements of context-dependent ribozyme activity

NY Alperovich, OB Vasilyeva, SW Schaffter - bioRxiv, 2024 - biorxiv.org
Self-cleaving ribozymes are important tools in synthetic biology, biomanufacturing, and
nucleic acid therapeutics. These broad applications deploy ribozymes in many genetic and …

[PDF][PDF] RNA-based portable genetic circuits and synthetic plasmids

B Liu - 2023 - repository.rice.edu
The lack of modularity impedes the creation of synthetic biological systems at the same level
of complexity as modern electronic systems, which can contain millions of logic gates …

Transcriptional control of the lacZ promoter is under directional and diversifying selection

M Vlková, OK Silander - bioRxiv, 2022 - biorxiv.org
Bacterial cells often respond to changes in the environment by modifying protein expression.
This can be achieved through changes in transcriptional or translational activity, or both …