Efficient genetic approaches for improvement of plasmid based expression of recombinant protein in Escherichia coli: A review

TK Singha, P Gulati, A Mohanty, YP Khasa… - Process …, 2017 - Elsevier
During the past two decades, there has been an explosion of new knowledge and
techniques in the field of recombinant protein expression. However, over-expression of …

Pyridine dinucleotides from molecules to man

JP Fessel, WM Oldham - Antioxidants & redox signaling, 2018 - liebertpub.com
Significance: Pyridine dinucleotides, nicotinamide adenine dinucleotide (NAD) and
nicotinamide adenine dinucleotide phosphate (NADP), were discovered more than 100 …

Cofactor specificity of glucose-6-phosphate dehydrogenase isozymes in Pseudomonas putida reveals a general principle underlying glycolytic strategies in bacteria

DC Volke, K Olavarría, PI Nikel - Msystems, 2021 - Am Soc Microbiol
ABSTRACT Glucose-6-phosphate dehydrogenase (G6PDH) is widely distributed in nature
and catalyzes the first committing step in the oxidative branch of the pentose phosphate (PP) …

Role of amino acid oxidation and protein unfolding in peroxyl radical and peroxynitrite-induced inactivation of glucose-6-phosphate dehydrogenase from Leuconostoc …

JD Figueroa, E Fuentes-Lemus, JS Reyes… - Free Radical Biology …, 2022 - Elsevier
The mechanisms underlying the inactivation of Leuconostoc mesenteroides glucose 6-
phosphate dehydrogenase (G6PDH) induced by peroxyl radicals (ROO●) and peroxynitrite …

Simultaneous observation of the anomerization and reaction rates of enzymatic dehydrogenation of Glucose-6-Phosphate by dissolution DNP

M Soussi-Therond, D Guarin… - Journal of the …, 2024 - ACS Publications
The hyperpolarization of biological samples using dissolution dynamic nuclear polarization
(dDNP) has become an attractive method for the monitoring of fast chemical and enzymatic …

Engineering an acetoacetyl-CoA reductase from Cupriavidus necator toward NADH preference under physiological conditions

K Olavarria, YO Pijman, R Cabrera… - Scientific Reports, 2022 - nature.com
The coupling of PHB generation with NADH reoxidation is required to generate PHB as a
fermentation product. A fundamental trait to accomplish this feature is to express a functional …

Growth Adaptation of gnd and sdhCB Escherichia coli Deletion Strains Diverges From a Similar Initial Perturbation of the Transcriptome

D McCloskey, S Xu, TE Sandberg, E Brunk… - Frontiers in …, 2018 - frontiersin.org
Adaptive laboratory evolution (ALE) has emerged as a new approach with which to pursue
fundamental biological inquiries and, in particular, new insights into the systemic function of …

Glucose-6-Phosphate Dehydrogenase, ZwfA, a Dual Cofactor-Specific Isozyme Is Predominantly Involved in the Glucose Metabolism of Pseudomonas bharatica …

BA Shah, ST Kasarlawar, PS Phale - Microbiology Spectrum, 2022 - Am Soc Microbiol
ABSTRACT Glucose-6-phosphate dehydrogenase (Zwf) is an important enzyme in glucose
metabolism via the Entner-Doudoroff pathway and the first enzyme in the oxidative pentose …

The Nitrogen Regulatory PII Protein (GlnB) and N-Acetylglucosamine 6-Phosphate Epimerase (NanE) Allosterically Activate Glucosamine 6-Phosphate Deaminase …

IA Rodionova, N Goodacre, M Babu, A Emili… - Journal of …, 2018 - Am Soc Microbiol
Amino sugars are good sources of both ammonia and fructose-6-phosphate, produced by
the glucosamine 6-phosphate deaminase, NagB. NagB is known to be allosterically …

[HTML][HTML] Engineering of increased L-Threonine production in bacteria by combinatorial cloning and machine learning

P Hanke, B Parrello, O Vasieva, C Akins… - Metabolic Engineering …, 2023 - Elsevier
The goal of this study is to develop a general strategy for bacterial engineering using an
integrated synthetic biology and machine learning (ML) approach. This strategy was …