Mechanisms of lncRNA biogenesis as revealed by nascent transcriptomics

T Nojima, NJ Proudfoot - Nature reviews Molecular cell biology, 2022 - nature.com
Mammalian genomes express two principal gene categories through RNA polymerase II-
mediated transcription: protein-coding transcription units and non-coding RNA transcription …

Knowing when to stop: Transcription termination on protein-coding genes by eukaryotic RNAPII

JB Rodríguez-Molina, S West, LA Passmore - Molecular cell, 2023 - cell.com
Gene expression is controlled in a dynamic and regulated manner to allow for the consistent
and steady expression of some proteins as well as the rapidly changing production of other …

3′-end processing of eukaryotic mRNA: machinery, regulation, and impact on gene expression

V Boreikaitė, LA Passmore - Annual review of biochemistry, 2023 - annualreviews.org
Formation of the 3′ end of a eukaryotic mRNA is a key step in the production of a mature
transcript. This process is mediated by a number of protein factors that cleave the pre …

Elevated pre-mRNA 3′ end processing activity in cancer cells renders vulnerability to inhibition of cleavage and polyadenylation

Y Cui, L Wang, Q Ding, J Shin, J Cassel, Q Liu… - Nature …, 2023 - nature.com
Cleavage and polyadenylation (CPA) is responsible for 3′ end processing of eukaryotic
poly (A)+ RNAs and preludes transcriptional termination. JTE-607, which targets CPSF-73 …

Widespread remodeling of the m6A RNA-modification landscape by a viral regulator of RNA processing and export

KP Srinivas, DP Depledge, JS Abebe… - Proceedings of the …, 2021 - National Acad Sciences
N 6-methyladenosine (m6A) is the most abundant internal messenger RNA (mRNA)
modification, contributing to the processing, stability, and function of methylated RNAs …

Translation—a tug of war during viral infection

B Rozman, T Fisher, N Stern-Ginossar - Molecular cell, 2023 - cell.com
Viral reproduction is contingent on viral protein synthesis that relies on the host ribosomes.
As such, viruses have evolved remarkable strategies to hijack the host translational …

HSV-1 DNA replication—coordinated regulation by viral and cellular factors

JE Packard, JA Dembowski - Viruses, 2021 - mdpi.com
DNA replication is an integral step in the herpes simplex virus type 1 (HSV-1) life cycle that
is coordinated with the cellular DNA damage response, repair and recombination of the viral …

Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression

NA Rosa-Mercado, JT Zimmer, M Apostolidi, J Rinehart… - Molecular cell, 2021 - cell.com
Stress-induced readthrough transcription results in the synthesis of downstream-of-gene
(DoG)-containing transcripts. The mechanisms underlying DoG formation during cellular …

SRSF3 and SRSF7 modulate 3′ UTR length through suppression or activation of proximal polyadenylation sites and regulation of CFIm levels

OD Schwich, N Blümel, M Keller, M Wegener, ST Setty… - Genome biology, 2021 - Springer
Background Alternative polyadenylation (APA) refers to the regulated selection of
polyadenylation sites (PASs) in transcripts, which determines the length of their 3 …

mRNA 3'UTR lengthening by alternative polyadenylation attenuates inflammatory responses and correlates with virulence of Influenza A virus

V Bergant, D Schnepf, N de Andrade Krätzig… - Nature …, 2023 - nature.com
Abstract Changes of mRNA 3'UTRs by alternative polyadenylation (APA) have been
associated to numerous pathologies, but the mechanisms and consequences often remain …