Alternative splicing is a substantial contributor to the high complexity of transcriptomes of
multicellular eukaryotes. In this Review, we discuss the accumulated evidence that most of …

Recent advances in sequencing techniques that measure nascent transcripts and that
reveal the positioning of RNA polymerase II (Pol II) have shown that the pausing of Pol II in …

Protein lysine acetylation has emerged as a key posttranslational modification in cellular
regulation, in particular through the modification of histones and nuclear transcription …

Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA
polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the …

Alternative splicing was discovered simultaneously with splicing over three decades ago.
Since then, an enormous body of evidence has demonstrated the prevalence of alternative …

The nucleoplasm is not homogenous; it consists of many types of nuclear bodies, also
known as nuclear domains or nuclear subcompartments. These self-organizing structures …

Alternative splicing of pre-mRNA is a prominent mechanism to generate protein diversity, yet
its regulation is poorly understood. We demonstrated a direct role for histone modifications …

Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

Alternative splicing plays critical roles in differentiation, development, and disease and is a
major source for protein diversity in higher eukaryotes. Analysis of alternative splicing …

Production of mRNA depends critically on the rate of RNA polymerase II (Pol II) elongation.
To dissect Pol II dynamics in mouse ES cells, we inhibited Pol II transcription at either …