Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

Increasing the temperature by just a few degrees may lead to structural perturbation or
unfolding of the protein and consequent loss of function. The concepts of flexibility and …

De novo protein design for catalysis of any desired chemical reaction is a long-standing goal
in protein engineering because of the broad spectrum of technological, scientific and …

Designing catalysts that achieve the rates and selectivities of natural enzymes is a long-
standing goal in protein chemistry. Here, we show that an ultrahigh-throughput droplet …

Directed evolution circumvents our profound ignorance of how a protein's sequence
encodes its function by using iterative rounds of random mutation and artificial selection to …

The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

2017-08-22 Assigned to MUSE BIOTECHNOLOGY, INC. reassignment MUSE
BIOTECHNOLOGY, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT …

Saturation mutagenesis probes define sections of the vast protein sequence space.
However, even if randomization is limited this way, the combinatorial numbers problem is …

Highlights•Enzyme activity/stability can be improved upon immobilization on nanoparticles
(NPs).•Five underlying physicochemical mechanisms describe enzyme–NP activity.•Carbon …

2018-02-08 Assigned to THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY
CORPORATE reassignment THE REGENTS OF THE UNIVERSITY OF COLORADO, A …