Membrane curvature is no longer seen as a passive consequence of cellular activity but an
active means to create membrane domains and to organize centres for membrane …

Biological membranes exhibit various function-related shapes, and the mechanism by which
these shapes are created is largely unclear. Here, we classify possible curvature-generating …

Amphiphilic molecules, of which soap is a typical example, possess antagonistic hydrophilic
and hydrophobic moieties in the same molecule. Carbohydrate amphiphiles, commonly …

Since the advent of the green fluorescent protein, the subcellular localization, mobility,
transport routes and binding interactions of proteins can be studied in living cells. Live cell …

Coat protein complex I (COPI) and COPII are required for bidirectional membrane trafficking
between the endoplasmic reticulum (ER) and the Golgi. While these core coat machineries …

Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-
substituted normal rat kidney cells have been made by dual-axis, high-voltage EM …

Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has
established itself as a key technology to study protein-protein interactions in living cells. A …

Dynamic molecular interactions are fundamental to all cellular processes. In vivo analyses of
these interactions are frequently done using fluorescence recovery after photobleaching …

▪ Abstract Green fluorescent protein chimerae acting as reporters for protein localization and
trafficking within the secretory membrane system of living cells have been used in a wide …

Plants are exposed to a wide range of potential pathogens, which derive from diverse phyla.
Therefore, plants have developed successful defense mechanisms during co-evolution with …