λ recombination and recombineering

KC Murphy - EcoSal Plus, 2016‏ - journals.asm.org
The bacteriophage λ Red homologous recombination system has been studied over the
past 50 years as a model system to define the mechanistic details of how organisms …

Bacterial genetic engineering by means of recombineering for reverse genetics

U Fels, K Gevaert, P Van Damme - Frontiers in Microbiology, 2020‏ - frontiersin.org
Serving a robust platform for reverse genetics enabling the in vivo study of gene functions
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …

Obg and membrane depolarization are part of a microbial bet-hedging strategy that leads to antibiotic tolerance

N Verstraeten, WJ Knapen, CI Kint, V Liebens… - Molecular cell, 2015‏ - cell.com
Within bacterial populations, a small fraction of persister cells is transiently capable of
surviving exposure to lethal doses of antibiotics. As a bet-hedging strategy, persistence …

[HTML][HTML] Prophages encode phage-defense systems with cognate self-immunity

SV Owen, N Wenner, CL Dulberger, EV Rodwell… - Cell host & …, 2021‏ - cell.com
Temperate phages are pervasive in bacterial genomes, existing as vertically inherited
islands termed prophages. Prophages are vulnerable to predation of their host bacterium by …

Activation of PmrA inhibits LpxT‐dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides

CM Herrera, JV Hankins, MS Trent - Molecular microbiology, 2010‏ - Wiley Online Library
During its transport to the bacterial surface, the phosphate groups of the lipid A anchor of
Escherichia coli and Salmonella lipopolysaccharide are modified by membrane enzymes …

Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms

LP Nedialkova, R Denzler, MB Koeppel, M Diehl… - PLoS …, 2014‏ - journals.plos.org
The host's immune system plays a key role in modulating growth of pathogens and the
intestinal microbiota in the gut. In particular, inflammatory bowel disorders and pathogen …

Relation between tetR and tetA expression in tetracycline resistant Escherichia coli

TSB Møller, M Overgaard, SS Nielsen, V Bortolaia… - BMC microbiology, 2016‏ - Springer
Background Tetracyclines are among the most used antibiotics in livestock worldwide.
Resistance is widely disseminated in Escherichia coli, where it is generally mediated by …

Tunable hybrid carbon metabolism coordination for the carbon-efficient biosynthesis of 1, 3-butanediol in Escherichia coli

J Wang, R Zhang, J Zhang, X Gong, T Jiang, X Sun… - Green …, 2021‏ - pubs.rsc.org
Microbial-based bio-refining seeks to replace fossil-based chemical industry with reduced
capital cost and lower environmental concern. However, current metabolic engineering …

De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

J Wang, Y Wu, X Sun, Q Yuan, Y Yan - ACS synthetic biology, 2017‏ - ACS Publications
Microbial based bioplastics are promising alternatives to petroleum based synthetic plastics
due to their renewability and economic feasibility. Glutarate is one of the most potential …

A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum

FD Fabiani, TT Renault, B Peters, T Dietsche… - PLoS …, 2017‏ - journals.plos.org
Many bacteria move using a complex, self-assembling nanomachine, the bacterial
flagellum. Biosynthesis of the flagellum depends on a flagellar-specific type III secretion …