Employment of polysaccharides in enzyme immobilization

A Sharma, KS Thatai, T Kuthiala, G Singh… - Reactive and Functional …, 2021 - Elsevier
Analogous to free enzymes, immobilized enzymes are sturdy and resistant to environmental
alterations. With unmatched high catalytic activity, turnover number, and selectivity, enzymes …

Lipases for targeted industrial applications, focusing on the development of biotechnologically significant aspects: A comprehensive review of recent trends in protein …

N Vardar-Yel, HE Tütüncü, Y Sürmeli - International Journal of Biological …, 2024 - Elsevier
Lipases are remarkable biocatalysts, adept at catalyzing the breakdown of diverse
compounds into glycerol, fatty acids, and mono-and di-glycerides via hydrolysis. Beyond …

Enhanced catalytic performance of thermophilic GH11 xylanase by fusing carbohydrate-binding module 9-2 and linker for better synergistic degradation of wheat bran

Y Li, W Song, X Yin, S Rao, Q Zhang, J Zhou, J Li… - Process …, 2022 - Elsevier
In this study, the catalytic activity of xynAm1 (a thermophilic xylanase family 11 variant) was
significantly enhanced by fusing a carbohydrate-binding module (CBM) 9–2. First, CBM9–2 …

Rational and random mutagenesis of GDEst-95 carboxylesterase: New functionality insights

V Malunavicius, L Vaskevicius, A Gusaite… - International Journal of …, 2024 - Elsevier
Lipolytic enzymes are important contributors in industrial processes from lipid hydrolysis to
biofuel production or even polyester biodegradation. While these enzymes can be used in …

Chimeric versus isolated proteins: Biochemical characterization of the NADP+-dependent formate dehydrogenase from Pseudomonas sp. 101 fused with the Baeyer …

E Lio, P Parshin, E D'Oronzo, S Plebani… - International Journal of …, 2023 - Elsevier
The expression of multifunctional proteins can facilitate the setup of a biotechnology process
that requires multiple functions absolved by different proteins. Herein the functional and …

Immobilized GDEst-95, GDEst-lip and GD-95RM lipolytic enzymes for continuous flow hydrolysis and transesterification reactions

A Savickaite, M Sadauskas, R Gudiukaite - International Journal of …, 2021 - Elsevier
In this study lipolytic biocatalysts GD-95RM, GDEst-95 and GDEst-lip were immobilized by
encapsulation in calcium alginate beads. All three immobilized biocatalysts demonstrated …

Exploring the strategy of fusing sucrose synthase to glycosyltransferase UGT76G1 in enzymatic biotransformation

Y Tao, P Sun, R Cai, Y Li, H Jia - Applied Sciences, 2022 - mdpi.com
Uridine diphosphate glycosyltransferases (UGTs) as fine catalysts of glycosylation are
increasingly used in the synthesis of natural products. Sucrose synthase (SuSy) is …